The selective PAC1 receptor agonist maxadilan inhibits neurogenic vasodilation and edema formation in the mouse skin

E. Banki, Zs Hajna, A. Kemeny, B. Botz, P. Nagy, K. Bölcskei, G. Tóth, D. Reglodi, Z. Helyes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have earlier shown that PACAP-38 decreases neurogenic inflammation. However, there were no data on its receptorial mechanism and the involvement of its PAC1 and VPAC1/2 receptors (PAC1R, VPAC1/2R) in this inhibitory effect. Neurogenic inflammation in the mouse ear was induced by topical application of the Transient Receptor Potential Ankyrin 1 (TRPA1) receptor activator mustard oil (MO). Consequent neurogenic edema, vasodilation and plasma leakage were assessed by measuring ear thickness with engineer's micrometer, detecting tissue perfusion by laser Doppler scanning and Evans blue or indocyanine green extravasation by intravital videomicroscopy or fluorescence imaging, respectively. Myeloperoxidase activity, an indicator of neutrophil infiltration, was measured from the ear homogenates with spectrophotometry. The selective PAC1R agonist maxadilan, the VPAC1/2R agonist vasoactive intestinal polypeptide (VIP) or the vehicle were administered i.p. 15 min before MO. Substance P (SP) concentration of the ear was assessed by radioimmunoassay. Maxadilan significantly diminished MO-induced neurogenic edema, increase of vascular permeability and vasodilation. These inhibitory effects of maxadilan may be partially due to the decreased substance P (SP) levels. In contrast, inhibitory effect of VIP on ear swelling was moderate, without any effect on MO-induced plasma leakage or SP release, however, activation of VPAC1/2R inhibited the increased microcirculation caused by the early arteriolar vasodilation. Neither the PAC1R, nor the VPAC1/2R agonist influenced the MO-evoked increase in tissue myeloperoxidase activity. These results clearly show that PAC1R activation inhibits acute neurogenic arterial vasodilation and plasma protein leakage from the venules, while VPAC1/2R stimulation is only involved in the attenuation of vasodilation.

Original languageEnglish
Pages (from-to)538-547
Number of pages10
JournalNeuropharmacology
Volume85
DOIs
Publication statusPublished - 2014

Fingerprint

Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Vasodilation
Ear
Edema
Substance P
Skin
Neurogenic Inflammation
Vasoactive Intestinal Peptide
Peroxidase
Receptors, Vasoactive Intestinal Polypeptide, Type I
Ankyrins
Pituitary Adenylate Cyclase-Activating Polypeptide
Video Microscopy
Evans Blue
Indocyanine Green
Venules
Neutrophil Infiltration
Spectrophotometry
Optical Imaging
Capillary Permeability

Keywords

  • Maxadilan
  • Neurogenic inflammation
  • PACAP
  • Plasma leakage
  • Vasodilation
  • VIP

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology
  • Medicine(all)

Cite this

The selective PAC1 receptor agonist maxadilan inhibits neurogenic vasodilation and edema formation in the mouse skin. / Banki, E.; Hajna, Zs; Kemeny, A.; Botz, B.; Nagy, P.; Bölcskei, K.; Tóth, G.; Reglodi, D.; Helyes, Z.

In: Neuropharmacology, Vol. 85, 2014, p. 538-547.

Research output: Contribution to journalArticle

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AU - Hajna, Zs

AU - Kemeny, A.

AU - Botz, B.

AU - Nagy, P.

AU - Bölcskei, K.

AU - Tóth, G.

AU - Reglodi, D.

AU - Helyes, Z.

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