The Schizosaccharomyces pombe genes sep10 and sep11 encode putative general transcriptional regulators involved in multiple cellular processes

Z. Szilagyi, A. Grallert, N. Nemeth, M. Sipiczki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have previously described the genetic analysis of eleven complementation groups (sep6-sep16) defined by Schizosaccharomyces pombe mutants that are defective in cell separation and sexual differentiation. Here we report on the cloning and characterisation of two members of this set, sep10 and sep11. Sequencing of the full-length sep10 revealed a continuous ORF that encodes a conserved protein with possible functions in general transcriptional regulation. The coding region of sep11 is interrupted by introns and the putative sep11 protein shows no sequence similarity with known proteins of other species. Disruption of each gene causes temperature sensitivity. Simultaneous disruption of both genes is lethal, demonstrating that sep10 and sep11 perform related, overlapping functions. Overexpression of aff1/ste11, a pivotal regulator of sexual development, suppresses the sterility of sep10- cells, which suggests that sep10 is needed for the activity of aff1/ste11.

Original languageEnglish
Pages (from-to)553-562
Number of pages10
JournalMolecular Genetics and Genomics
Volume268
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Schizosaccharomyces
Lethal Genes
Genes
Sex Differentiation
Sexual Development
Proteins
Cell Separation
Introns
Infertility
Open Reading Frames
Organism Cloning
Cell Differentiation
Temperature

Keywords

  • Cytokinesis
  • Fission yeast
  • Mediator
  • sep
  • Sexual differentiation

ASJC Scopus subject areas

  • Genetics

Cite this

The Schizosaccharomyces pombe genes sep10 and sep11 encode putative general transcriptional regulators involved in multiple cellular processes. / Szilagyi, Z.; Grallert, A.; Nemeth, N.; Sipiczki, M.

In: Molecular Genetics and Genomics, Vol. 268, No. 4, 2002, p. 553-562.

Research output: Contribution to journalArticle

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