The Salvage of Deoxycytidine into dCDP-Diacylglycerol by Macrophages and Lymphocytes

A. Hrabák, T. Spasokukotskaja, A. Temesi, M. Staub

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Abstract

Extracellular deoxycytidine (CdR) was previously shown to be salvaged into water soluble [1] and also into lipidic [2] precursors of phospholipids in stimulated lymphocytes and in lymphoma cells [3]. In this paper we have described that non-dividing murine macrophages salvaged not only 5-3H-CdR but also tritiated thymidine(3H-TdR) mainly into the pools as nucleotides. Chlorprosazine shifted the CdR salvage into a lipidic compound of the cells which was identified as 3H-dCDP-diacylglycerol (dCDP-DAG). After 5-3H-CdR labeling the lipid/DNA ratio was eleven times higher in macrophages than in tonsillar lymphocytes. Thin layer chromatography (TLC) on borate impregnated silica gel plates gave clear separation of CDP-DAG from dCDP-DAG supporting that the extracellular precursor for it is exclusively deoxycytidine and not ribocytidine. No interconversion between deoxy- and ribocytidine could be observed neither in lymphocytes nor in macrophages.

Original languageEnglish
Pages (from-to)212-219
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number1
DOIs
Publication statusPublished - May 28 1993

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Salvaging
Deoxycytidine
Lymphocytes
Macrophages
Diglycerides
Cytidine Diphosphate
Thin layer chromatography
Borates
Silica Gel
Thin Layer Chromatography
Thymidine
Labeling
Lymphoma
Phospholipids
Nucleotides
Lipids
Water
DNA
2'-deoxycytidine diphosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

The Salvage of Deoxycytidine into dCDP-Diacylglycerol by Macrophages and Lymphocytes. / Hrabák, A.; Spasokukotskaja, T.; Temesi, A.; Staub, M.

In: Biochemical and Biophysical Research Communications, Vol. 193, No. 1, 28.05.1993, p. 212-219.

Research output: Contribution to journalArticle

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