Extracellular deoxycytidine (CdR) was previously shown to be salvaged into water soluble  and also into lipidic  precursors of phospholipids in stimulated lymphocytes and in lymphoma cells . In this paper we have described that non-dividing murine macrophages salvaged not only 5-3H-CdR but also tritiated thymidine(3H-TdR) mainly into the pools as nucleotides. Chlorprosazine shifted the CdR salvage into a lipidic compound of the cells which was identified as 3H-dCDP-diacylglycerol (dCDP-DAG). After 5-3H-CdR labeling the lipid/DNA ratio was eleven times higher in macrophages than in tonsillar lymphocytes. Thin layer chromatography (TLC) on borate impregnated silica gel plates gave clear separation of CDP-DAG from dCDP-DAG supporting that the extracellular precursor for it is exclusively deoxycytidine and not ribocytidine. No interconversion between deoxy- and ribocytidine could be observed neither in lymphocytes nor in macrophages.
|Number of pages||8|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - May 28 1993|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology