The roles of the α1-adrenergic receptor subtypes in rat embryonic implantation

Eszter Ducza, Róbert Gáspár, Attila Mihályi, Zsolt Kormányos, George Falkay

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To focus on the possible roles of α1-adrenergic receptors (α1-ARs) in rat embryonic implantation. Design: Laboratory study. Setting: Animal and pharmacology laboratory at Department of Pharmacodynamics and Biopharmacy, University of Szeged, Hungary. Animal(s): Pregnant and nonpregnant Sprague-Dawley rats. Intervention(s): Uterus tissues were collected during the peri-implantation period. Main Outcome Measure(s): We used a reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting to demonstrate the expressions of mRNAs and the protein expressions of the α1-AR subtypes in the early-pregnant uterus. Electric field stimulation was applied to test the pharmacologic reactivity of the α1A-AR, and the physiologic role of this receptor was tested in a knock-down transformed animal model using an antisense oligonucleotide that elicits sequence-selective inhibition of the α1A-AR gene expression. Result(s): The presence of all α1-AR subtypes (α1A, α1B, and α1D) was proved, with a predominance of α1A-AR. The maximal expression of the α1A-AR was attained on the day of implantation. The selective α1A antagonist 5-methylurapidil inhibited the contraction in a dose-dependent manner. The number of implantation sites was decreased (∼75%) in the α1A-AR knock-down transformed rats. Conclusion(s): We assume that the α1A-AR predominance plays a crucial role in embryonic implantation in the rat.

Original languageEnglish
Pages (from-to)1224-1229
Number of pages6
JournalFertility and Sterility
Volume91
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

Keywords

  • antisense oligonucleotide
  • implantation
  • rat
  • α-adrenergic receptors

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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