The role of uron and chlorobenzene derivatives, as potential endocrine disrupting compounds, in the secretion of ACTH and PRL

Krisztian Sepp, Anna M. Laszlo, Z. Molnár, Andrea Serester, Tunde Alapi, M. Gálfi, Zsuzsanna Valkusz, Marianna Radacs

Research output: Contribution to journalArticle

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Abstract

Uron herbicides polluting the environment represent a serious concern for environmental health and may be regarded as endocrine-disrupting compounds (EDCs), which influence the regulation of human homeostasis. We aimed to investigate the effect of EDC urons (phenuron: PU, monuron: MU, and diuron: DU) and chlorobenzenes on the basal release of the adrenocorticotropic hormone (ACTH), which is a part of the adenohypophysis-adrenocortical axis. Hormone secretion in the presence of EDC was studied in two cell types: normal adenohypophysis cells (AdH) and cells of prolactinomas (PRLOMA). PRLOMA was induced in female Wistar rats by subcutaneously injecting them with estrone acetate for 6 months. AdH and PRLOMA were separated from treated and untreated experimental animals, dissociated enzymatically and mechanically in order to create monolayer cell cultures, which served as an experimental in vitro model. We investigated the effects of ED agents separately and in combination on ACTH and prolactin (PRL) release through the hypophyseal-adrenal axis. Hormone determination was carried out by the luminescent immunoassay and the radioimmunoassay methods. Our results showed that (1) uron agents separately did not change ACTH and PRL release in AdH culture; (2) ACTH secretion in arginine vasopressin-(AVP-) activated AdH cells was significantly increased by EDC treatment; (3) ED agents increased the basal hormone release (ACTH, PRL) in PRLOMA cells; and (4) EDC exposure increased ACTH release in AVP-activated PRLOMA cells. We conclude that the herbicides PU, MU, and DU carry EDC effects and show human toxicity potential.

Original languageEnglish
Article number7493418
JournalInternational Journal of Endocrinology
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Prolactin
Adrenocorticotropic Hormone
Prolactinoma
Anterior Pituitary Gland
Herbicides
Hormones
Chlorobenzenes
Cell Culture Techniques
Diuron
Environmental Health
chlorobenzene
Arginine Vasopressin
Immunoassay
Radioimmunoassay
Wistar Rats
Homeostasis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems

Cite this

The role of uron and chlorobenzene derivatives, as potential endocrine disrupting compounds, in the secretion of ACTH and PRL. / Sepp, Krisztian; Laszlo, Anna M.; Molnár, Z.; Serester, Andrea; Alapi, Tunde; Gálfi, M.; Valkusz, Zsuzsanna; Radacs, Marianna.

In: International Journal of Endocrinology, Vol. 2018, 7493418, 01.01.2018.

Research output: Contribution to journalArticle

Sepp, Krisztian ; Laszlo, Anna M. ; Molnár, Z. ; Serester, Andrea ; Alapi, Tunde ; Gálfi, M. ; Valkusz, Zsuzsanna ; Radacs, Marianna. / The role of uron and chlorobenzene derivatives, as potential endocrine disrupting compounds, in the secretion of ACTH and PRL. In: International Journal of Endocrinology. 2018 ; Vol. 2018.
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