The role of the inhibition of glutathione-s-transferase in the protective mechanisms of ischemic postconditioning

Borbála Balatonyi, B. Gasz, Viktória Kovács, J. Lantos, Gábor Jancsó, Nándor Marczin, E. Rőth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The antioxidant glutathione-S-transferase (GST) is a crucial determinant of the development of ischaemic-reperfusion (I/R) injury, and plays a pivotal role in the regulation of the mitogen activated protein kinase (MAPK) pathways involved in stress response and apoptosis. The aim of this study was to investigate whether inhibition of GST can abolish the benefit of ischaemic postconditioning (IPoC). A neonatal rat cardiomyocyte cell culture was prepared and divided into 6 groups: (I) control group without treatment; (II) cells exposed to simulated I/R; (III) simulated I/R (sI/R) with IPoC; (IV) ethacrynic acid (EA) alone; (V) sI/R with EA; and (VI) sI/R and IPoC together with EA. Viability of the cells was measured by MTT assay, the quantity of apoptotic cells was assessed by flow cytometry following annexin V-FITC - propidium-iodide double staining. The activation of JNK, p38, ERK/p42-p44 MAPKs, and GSK-3 protein kinase was determined by flow-cytometric assay. GST inhibition markedly increased the apoptosis and decreased the cell viability despite IPoC. The protective effect of IPoC was lost in GST-inhibited groups for all MAPKs and GSK-3. GST activity is required for the survival of cultured cardiomyocytes under stress conditions. GST inhibition was associated with differential activation of MAP and the protein kinases regulating these pathways in the process of ischaemic postconditioning.

Original languageEnglish
Pages (from-to)625-632
Number of pages8
JournalCanadian Journal of Physiology and Pharmacology
Volume91
Issue number8
DOIs
Publication statusPublished - 2013

Fingerprint

Ischemic Postconditioning
Glutathione Transferase
Ethacrynic Acid
Glycogen Synthase Kinase 3
Cardiac Myocytes
Protein Kinases
Reperfusion
Cell Survival
Apoptosis
Mitogen-Activated Protein Kinase 3
Propidium
Fluorescein-5-isothiocyanate
Annexin A5
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinases
Reperfusion Injury
Flow Cytometry
Cell Culture Techniques
Antioxidants
Staining and Labeling

Keywords

  • Apoptosis
  • Cell viability
  • Ethacrynic acid (ea)
  • Glutathione-s-transferase (gst)
  • Ischaemic postconditioning (ipoc)
  • Mitogen activated protein kinase (mapk)
  • Primer cardiomyocyte cell culture

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology

Cite this

The role of the inhibition of glutathione-s-transferase in the protective mechanisms of ischemic postconditioning. / Balatonyi, Borbála; Gasz, B.; Kovács, Viktória; Lantos, J.; Jancsó, Gábor; Marczin, Nándor; Rőth, E.

In: Canadian Journal of Physiology and Pharmacology, Vol. 91, No. 8, 2013, p. 625-632.

Research output: Contribution to journalArticle

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