The role of the FtsH protease in the repair of photosystem II after damage by UV-B radiation in Synechocytis 6803

Otilia Cheregi, Cosmin Sicora, P. Kós, Peter J. Nixon, I. Vass

Research output: Contribution to journalArticle

Abstract

We have investigated the repair mechanism of UV-damaged PSII in Synechocystis 6803 cells, in relation to the role of the FtsH protease. FtsH is an ATP- dependent metalloprotease that belongs to the AAA- (ATP-ase associated with diverse cellular activities) protein family. One of the homologues is encoded by the slr0228 gene whose transcript level is induced 16 fold by UV-B radiation. The UV-B induced loss of oxygen evolving activity was accelerated in the ΔFtsH mutant, which lacks the FtsH protease due to the deletion of the slr0228 gene. In the presence of the protein synthesis inhibitor lincomycin, the rate of UV-B induced oxygen inhibition in the ΔFtsH mutant show the same kinetics as in the absence of antibiotic. In addition, the mutation completely abolished the restoration of oxygen evolution in visible light following UV-B treatment. These data demonstrate that PSII repair is blocked in the absence of the FtsH protease both under UV illumination and in visible light following the UV-B treatment.

Original languageEnglish
Pages (from-to)199-201
Number of pages3
JournalActa Biologica Szegediensis
Volume49
Issue number1-2
Publication statusPublished - 2005

Fingerprint

Photosystem II Protein Complex
photosystem II
ultraviolet radiation
Repair
Peptide Hydrolases
proteinases
Radiation
Oxygen
Genes
Adenosine Triphosphate
Lincomycin
Synechocystis
Light
Protein Synthesis Inhibitors
Gene Deletion
Metalloproteases
Lighting
Ultraviolet radiation
Restoration
Synechocystis sp. PCC 6803

Keywords

  • FtsH protease
  • Lincomycin
  • Oxygen revolution
  • PSII repair
  • UV-B radiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Neuroscience(all)
  • Applied Microbiology and Biotechnology

Cite this

The role of the FtsH protease in the repair of photosystem II after damage by UV-B radiation in Synechocytis 6803. / Cheregi, Otilia; Sicora, Cosmin; Kós, P.; Nixon, Peter J.; Vass, I.

In: Acta Biologica Szegediensis, Vol. 49, No. 1-2, 2005, p. 199-201.

Research output: Contribution to journalArticle

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