The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division

Z. Magyar, Lieven De Veylder, Ana Atanassova, László Bakó, Dirk Inzé, László Bögre

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The molecular mechanisms by which the phytohormone auxin coordinates cell division with cell growth and differentiation are largely unknown. Here, we show that in Arabidopsis thaliana E2FB, accumulation and stability are positively regulated by auxin. Coexpression of E2FB, but not of E2FA, with its dimerization partner A, stimulated cell proliferation in the absence of auxin in tobacco (Nicotiana tabacum) Bright Yellow-2 cells. E2FB regulated the entry into both S- and M-phases, the latter corresponding to the activation of a plant-specific mitotic regulator, CDKB1;1. Increased E2FB levels led to shortened cell cycle duration, elevated cell numbers, and extremely small cell sizes. In the absence of auxin, cells elongated with concomitant increase in their ploidy level, but both were strongly inhibited by E2FB. We conclude that E2FB is one of the key targets for auxin to determine whether cells proliferate or whether they exit the cell cycle, enlarge, and endoreduplicate their DNA.

Original languageEnglish
Pages (from-to)2527-2541
Number of pages15
JournalPlant Cell
Volume17
Issue number9
DOIs
Publication statusPublished - 2005

Fingerprint

Indoleacetic Acids
Arabidopsis
Cell Division
cell division
auxins
Transcription Factors
transcription factors
Cells
Tobacco
cells
cell cycle
Cell Cycle
Plant Growth Regulators
dimerization
Dimerization
Ploidies
Cell proliferation
Cell growth
S Phase
Cell Size

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. / Magyar, Z.; De Veylder, Lieven; Atanassova, Ana; Bakó, László; Inzé, Dirk; Bögre, László.

In: Plant Cell, Vol. 17, No. 9, 2005, p. 2527-2541.

Research output: Contribution to journalArticle

Magyar, Z, De Veylder, L, Atanassova, A, Bakó, L, Inzé, D & Bögre, L 2005, 'The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division', Plant Cell, vol. 17, no. 9, pp. 2527-2541. https://doi.org/10.1105/tpc.105.033761
Magyar, Z. ; De Veylder, Lieven ; Atanassova, Ana ; Bakó, László ; Inzé, Dirk ; Bögre, László. / The role of the Arabidopsis E2FB transcription factor in regulating auxin-dependent cell division. In: Plant Cell. 2005 ; Vol. 17, No. 9. pp. 2527-2541.
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