The role of the acquisition methods in the analysis of the non-steroidal anti-inflammatory drugs in Danube River by gas chromatography-mass spectrometry

A. Helenkár, Á Sebk, G. Záray, I. Molnár-Perl, A. Vasanits-Zsigrai

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In this paper authors describe a GC-MS acquisition study, relating to the most common, non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, naproxen, ketoprofen and diclofenac. As novelties to the field, for the trimethylsilyl (TMS) oxime ester derivatives of NSAIDs, at first, a tandem mass spectrometric (MS/MS) acquisition method has been developed, and, also for the first time, the three acquisition techniques, the full scan (FS), the selective ion monitoring (SIM) and the currently optimized MS/MS ones, have been compared: all three in parallel, under strictly the same derivatization/instrumental conditions, both from model solutions and from the Danube River samples. Critical evaluation of the three acquisition protocols was collated on their analytical performances and validated with the same characteristics like the six point calibration curve, the relative standard deviation percentages (RSD%) of parallel tests, the limit of quantitation (LOQ) and the instrumental limit of quantitation (ILQ) values. Data of six point calibration (r2 ≥ 0.997) and RSD% (average: 5.8 RSD%) values proved to be independent on the acquisition methods, while, LOQ and ILQ values furnished considerable differences. Decreasing LOQ data, (expressed in ng/L concentrations) were obtained in the FS, SIM, MS/MS line for ibuprofen (1.0, 0.43, 0.41), naproxen (1.1, 1.0, 0.42), ketoprofen (2.6, 1.0, 0.49) and diclofenac (1.4, 0.41, 0.21), respectively. The same trend was determined in terms of the ILQ values. The practical utility of the optimized MS/MS technique was confirmed by the quantitation of the NSAID contents of the Danube River samples, determined by all three acquisition techniques. Results obtained confirmed the primary importance of the MS/MS acquisition method, even in comparison to the SIM one: avoiding the extreme overestimation of the ibuprofen (≈100%) and ketoprofen (≈400%) concentrations in the Danube River samples.

Original languageEnglish
Pages (from-to)600-607
Number of pages8
JournalTalanta
Volume82
Issue number2
DOIs
Publication statusPublished - Jul 15 2010

Fingerprint

Ketoprofen
Ibuprofen
Rivers
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Naproxen
Anti-Inflammatory Agents
Diclofenac
Ions
Calibration
Monitoring
Pharmaceutical Preparations
Oximes
Esters
Derivatives

Keywords

  • Danube River
  • Drinking water
  • GC-MS FS
  • GC-MS SIM
  • GC-MS/MS
  • Mass fragmentation
  • Non-steroidal anti-inflammatory drugs

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

The role of the acquisition methods in the analysis of the non-steroidal anti-inflammatory drugs in Danube River by gas chromatography-mass spectrometry. / Helenkár, A.; Sebk, Á; Záray, G.; Molnár-Perl, I.; Vasanits-Zsigrai, A.

In: Talanta, Vol. 82, No. 2, 15.07.2010, p. 600-607.

Research output: Contribution to journalArticle

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