The role of sterility genes (ste and aff) in the initiation of sexual development in Schizosaccharomyces pombe

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Abstract

Haploid homothallic strains of Schizosaccharomyces pombe with mutations in any of nine "sterility genes" (ste) do not mate with wild-type fertile strains. Those defective in genes ste1 to ste4 and ste7 to ste9 are also deficient in meiosis and sporulation. I found that the ste1, ste3 and ste8 genes act very early in the sexual development, presumably before the pat1-controlled conjugation-specific event. ste5 and ste6 exert their function downstream of pat1 in the initiation of conjugation and do not play any role in the meiotic pathway. ste2, ste4, ste7 and ste9 are involved in both sexual pathways: they seem to act downstream of pat1 in conjugation but upstream of pat1 in the initiation of meiosis. A new gene, aff1, whose defective allele suppresses the pat1-114-provoked haploid sporulation and arrest of vegetative growth is also described. It is supposed that the aff1+ gene product participates in a cascade of regulatory events, as a factor antagonistic to pat1.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalMGG Molecular & General Genetics
Volume213
Issue number2-3
DOIs
Publication statusPublished - Aug 1988

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Sexual Development
Schizosaccharomyces
Infertility
Genes
Haploidy
Meiosis
Alleles
Mutation
Growth

Keywords

  • Conjugation
  • Fission yeast
  • Meiosis
  • Protoplast fusion
  • Sterility

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "The role of sterility genes (ste and aff) in the initiation of sexual development in Schizosaccharomyces pombe",
abstract = "Haploid homothallic strains of Schizosaccharomyces pombe with mutations in any of nine {"}sterility genes{"} (ste) do not mate with wild-type fertile strains. Those defective in genes ste1 to ste4 and ste7 to ste9 are also deficient in meiosis and sporulation. I found that the ste1, ste3 and ste8 genes act very early in the sexual development, presumably before the pat1-controlled conjugation-specific event. ste5 and ste6 exert their function downstream of pat1 in the initiation of conjugation and do not play any role in the meiotic pathway. ste2, ste4, ste7 and ste9 are involved in both sexual pathways: they seem to act downstream of pat1 in conjugation but upstream of pat1 in the initiation of meiosis. A new gene, aff1, whose defective allele suppresses the pat1-114-provoked haploid sporulation and arrest of vegetative growth is also described. It is supposed that the aff1+ gene product participates in a cascade of regulatory events, as a factor antagonistic to pat1.",
keywords = "Conjugation, Fission yeast, Meiosis, Protoplast fusion, Sterility",
author = "M. Sipiczki",
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AU - Sipiczki, M.

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N2 - Haploid homothallic strains of Schizosaccharomyces pombe with mutations in any of nine "sterility genes" (ste) do not mate with wild-type fertile strains. Those defective in genes ste1 to ste4 and ste7 to ste9 are also deficient in meiosis and sporulation. I found that the ste1, ste3 and ste8 genes act very early in the sexual development, presumably before the pat1-controlled conjugation-specific event. ste5 and ste6 exert their function downstream of pat1 in the initiation of conjugation and do not play any role in the meiotic pathway. ste2, ste4, ste7 and ste9 are involved in both sexual pathways: they seem to act downstream of pat1 in conjugation but upstream of pat1 in the initiation of meiosis. A new gene, aff1, whose defective allele suppresses the pat1-114-provoked haploid sporulation and arrest of vegetative growth is also described. It is supposed that the aff1+ gene product participates in a cascade of regulatory events, as a factor antagonistic to pat1.

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KW - Conjugation

KW - Fission yeast

KW - Meiosis

KW - Protoplast fusion

KW - Sterility

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