The role of Sigma-1 receptor in sex-specific heat shock response in an experimental rat model of renal ischaemia/reperfusion injury

Adam Hosszu, Zsuzsanna Antal, Apor Veres-Szekely, Lilla Lenart, Dora Bianka Balogh, Edgar Szkibinszkij, Lilla Illesy, Judit Hodrea, Nora F. Banki, L. Wágner, A. Vannay, Attila J. Szabo, Andrea Fekete

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously showed that female rats are more protected against renal ischaemia/reperfusion (I/R) injury than males, which is partly attributed to their more pronounced heat shock response. We recently described that Sigma-1 receptor (S1R) activation improves postischaemic survival and renal function. 17β-estradiol activates S1R, thus here we investigated the role of sex-specific S1R activation and heat shock response in severe renal I/R injury. Proximal tubular cells were treated with 17β-estradiol, which caused direct S1R activation and subsequent induction of heat shock response. Uninephrectomized female, male and ovariectomized female (Ovx) Wistar rats were subjected to 50-min renal ischaemia followed by 2 (T2) and 24 (T24) hours of reperfusion. At T24 renal functional, impairment was less severe and structural damage was less prominent in females versus males or Ovx. Postischaemic increase in S1R, pAkt, HSF-1, HSP72 levels were detected as early as at T2, while pHSP27 was elevated later at T24. Abundance of heat shock proteins was higher in healthy female rats and remained higher at T2 and T24 (female versus male or Ovx; resp.). We propose a S1R-dependent mechanism, which contributes to the relative renoprotection of females after I/R injury by enhancing the heat shock response.

Original languageEnglish
JournalTransplant International
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Heat-Shock Response
Reperfusion Injury
Theoretical Models
Kidney
Estradiol
Heat-Shock Proteins
sigma-1 receptor
Reperfusion
Wistar Rats
Ischemia

Keywords

  • Heat shock proteins
  • Heat shock response
  • Kidney ischaemia/reperfusion
  • Oestrogen
  • Sex differences
  • Sigma-1 receptor

ASJC Scopus subject areas

  • Transplantation

Cite this

Hosszu, A., Antal, Z., Veres-Szekely, A., Lenart, L., Balogh, D. B., Szkibinszkij, E., ... Fekete, A. (Accepted/In press). The role of Sigma-1 receptor in sex-specific heat shock response in an experimental rat model of renal ischaemia/reperfusion injury. Transplant International. https://doi.org/10.1111/tri.13293

The role of Sigma-1 receptor in sex-specific heat shock response in an experimental rat model of renal ischaemia/reperfusion injury. / Hosszu, Adam; Antal, Zsuzsanna; Veres-Szekely, Apor; Lenart, Lilla; Balogh, Dora Bianka; Szkibinszkij, Edgar; Illesy, Lilla; Hodrea, Judit; Banki, Nora F.; Wágner, L.; Vannay, A.; Szabo, Attila J.; Fekete, Andrea.

In: Transplant International, 01.01.2018.

Research output: Contribution to journalArticle

Hosszu, A, Antal, Z, Veres-Szekely, A, Lenart, L, Balogh, DB, Szkibinszkij, E, Illesy, L, Hodrea, J, Banki, NF, Wágner, L, Vannay, A, Szabo, AJ & Fekete, A 2018, 'The role of Sigma-1 receptor in sex-specific heat shock response in an experimental rat model of renal ischaemia/reperfusion injury', Transplant International. https://doi.org/10.1111/tri.13293
Hosszu, Adam ; Antal, Zsuzsanna ; Veres-Szekely, Apor ; Lenart, Lilla ; Balogh, Dora Bianka ; Szkibinszkij, Edgar ; Illesy, Lilla ; Hodrea, Judit ; Banki, Nora F. ; Wágner, L. ; Vannay, A. ; Szabo, Attila J. ; Fekete, Andrea. / The role of Sigma-1 receptor in sex-specific heat shock response in an experimental rat model of renal ischaemia/reperfusion injury. In: Transplant International. 2018.
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AU - Lenart, Lilla

AU - Balogh, Dora Bianka

AU - Szkibinszkij, Edgar

AU - Illesy, Lilla

AU - Hodrea, Judit

AU - Banki, Nora F.

AU - Wágner, L.

AU - Vannay, A.

AU - Szabo, Attila J.

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