The role of phospholipids in regulating photosynthetic electron transport activities

Treatment of chloroplasts with phospholipase A2

G. Horváth, Magdolna Droppa, É. Hideg, Zsuzsanna Rózsa, T. Farkas

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13 Citations (Scopus)

Abstract

The involvement of phospholipids in the regulation of photosynthetic activities was studied by incubating isolated pea chloroplasts with phospholipase A2 to remove the β-positioned fatty acyl chains of the phospholipid molecules. The treatment was effective in eliminating 50% of phosphatidylglycerol (PG) and 90% of phosphatidylcholine (PCh) of the chloroplast thylakoids, but it resulted in only a partial decrease in photosynthetic electron transport activities. As found by fluorescence induction measurements the decrease in the electron flow activity was due to the inactivation of about 20% of photosystem II (PSII) centres. However, the fluorescence induction kinetics of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) poisoned chloroplasts showed that the number of PSIIβ-like centres was proportionally increased in the membrane by the progress of phospholipase A2 treatment, suggesting that phospholipid molecules may play an important role in regulating the association of peripheral chlorophyll a/b containing light-harvesting complex (LHCII-peripheral) with PSIIα. The specific involvement of PG and PCh in the organization and function of PSII is discussed.

Original languageEnglish
Pages (from-to)515-527
Number of pages13
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume3
Issue number4
DOIs
Publication statusPublished - 1989

Fingerprint

chloroplasts
phospholipase A2
Photosystem II Protein Complex
Phospholipases A2
Phospholipids
Chloroplasts
Electron Transport
photosystem II
electron transfer
phospholipids
Diuron
Phosphatidylglycerols
phosphatidylglycerols
induction
Fluorescence
Phosphatidylcholines
phosphatidylcholines
fluorescence
Molecules
electrons

Keywords

  • Chloroplast
  • fluorescence induction kinetics
  • lipid
  • phospholipase A
  • photosynthetic electron transport
  • thermoluminescence.

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

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title = "The role of phospholipids in regulating photosynthetic electron transport activities: Treatment of chloroplasts with phospholipase A2",
abstract = "The involvement of phospholipids in the regulation of photosynthetic activities was studied by incubating isolated pea chloroplasts with phospholipase A2 to remove the β-positioned fatty acyl chains of the phospholipid molecules. The treatment was effective in eliminating 50{\%} of phosphatidylglycerol (PG) and 90{\%} of phosphatidylcholine (PCh) of the chloroplast thylakoids, but it resulted in only a partial decrease in photosynthetic electron transport activities. As found by fluorescence induction measurements the decrease in the electron flow activity was due to the inactivation of about 20{\%} of photosystem II (PSII) centres. However, the fluorescence induction kinetics of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) poisoned chloroplasts showed that the number of PSIIβ-like centres was proportionally increased in the membrane by the progress of phospholipase A2 treatment, suggesting that phospholipid molecules may play an important role in regulating the association of peripheral chlorophyll a/b containing light-harvesting complex (LHCII-peripheral) with PSIIα. The specific involvement of PG and PCh in the organization and function of PSII is discussed.",
keywords = "Chloroplast, fluorescence induction kinetics, lipid, phospholipase A, photosynthetic electron transport, thermoluminescence.",
author = "G. Horv{\'a}th and Magdolna Droppa and {\'E}. Hideg and Zsuzsanna R{\'o}zsa and T. Farkas",
year = "1989",
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T1 - The role of phospholipids in regulating photosynthetic electron transport activities

T2 - Treatment of chloroplasts with phospholipase A2

AU - Horváth, G.

AU - Droppa, Magdolna

AU - Hideg, É.

AU - Rózsa, Zsuzsanna

AU - Farkas, T.

PY - 1989

Y1 - 1989

N2 - The involvement of phospholipids in the regulation of photosynthetic activities was studied by incubating isolated pea chloroplasts with phospholipase A2 to remove the β-positioned fatty acyl chains of the phospholipid molecules. The treatment was effective in eliminating 50% of phosphatidylglycerol (PG) and 90% of phosphatidylcholine (PCh) of the chloroplast thylakoids, but it resulted in only a partial decrease in photosynthetic electron transport activities. As found by fluorescence induction measurements the decrease in the electron flow activity was due to the inactivation of about 20% of photosystem II (PSII) centres. However, the fluorescence induction kinetics of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) poisoned chloroplasts showed that the number of PSIIβ-like centres was proportionally increased in the membrane by the progress of phospholipase A2 treatment, suggesting that phospholipid molecules may play an important role in regulating the association of peripheral chlorophyll a/b containing light-harvesting complex (LHCII-peripheral) with PSIIα. The specific involvement of PG and PCh in the organization and function of PSII is discussed.

AB - The involvement of phospholipids in the regulation of photosynthetic activities was studied by incubating isolated pea chloroplasts with phospholipase A2 to remove the β-positioned fatty acyl chains of the phospholipid molecules. The treatment was effective in eliminating 50% of phosphatidylglycerol (PG) and 90% of phosphatidylcholine (PCh) of the chloroplast thylakoids, but it resulted in only a partial decrease in photosynthetic electron transport activities. As found by fluorescence induction measurements the decrease in the electron flow activity was due to the inactivation of about 20% of photosystem II (PSII) centres. However, the fluorescence induction kinetics of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) poisoned chloroplasts showed that the number of PSIIβ-like centres was proportionally increased in the membrane by the progress of phospholipase A2 treatment, suggesting that phospholipid molecules may play an important role in regulating the association of peripheral chlorophyll a/b containing light-harvesting complex (LHCII-peripheral) with PSIIα. The specific involvement of PG and PCh in the organization and function of PSII is discussed.

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