The role of p53 in the induction of polyploidity of myelomonocytic leukemic M1/2 cells

Amnon Peled, Dov Schwartz, N. Barry Elkind, Roland Wolkowicz, Runzhao Li, Varda Rotter

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Abstract

p53 was shown to play a central role in the maintenance of genomic integrity. The present experiments suggest that p53 is involved in the control of cell ploidity. Using a p53 non-producer cell line, M1/2, that was reconstituted to express either wild type or mutant p53 protein, by infection with the temperature sensitive (Ts) p53 Val135 virus, it was found that both loss of wild type p53 or overexpression of mutant p53, may be associated with the generation of cell polyploidity. Overexpression of mutant p53 protein enhanced the appearance of giant cells that further accumulated following γ-irradiation. Expression of wild type p53 reduced the level of giant cells which accumulated in the parental M1/2 p53 nonproducer cells following γ-irradiation. This activity of the wild type p53 seems to be mediated by either the reduction in the rate of giant cell generation, as observed in M1/2 derived cell lines expressing low levels of wild type p53 protein or by facilitating their apoptosis, as observed in wild type p53 high-producer cells. The latter conclusion is further supported by the observation that isolated giant cells are directly induced to undergo apoptosis following wild type p53 expression.

Original languageEnglish
Pages (from-to)1677-1685
Number of pages9
JournalOncogene
Volume13
Issue number8
Publication statusPublished - Nov 20 1996

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Keywords

  • Giant cells
  • Mutant p53
  • Ploidity
  • Wild type p53

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Peled, A., Schwartz, D., Elkind, N. B., Wolkowicz, R., Li, R., & Rotter, V. (1996). The role of p53 in the induction of polyploidity of myelomonocytic leukemic M1/2 cells. Oncogene, 13(8), 1677-1685.