The role of over-pressured-layer chromatography among chromatographic methods for the determination of the aflatoxin content of fish

Eszter Papp, Boglárka Bagócsi, Klára H-Otta, Loréna Kovacsics-Ács, Emil Mincsovics

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aflatoxins are a family of toxic and carcinogenic metabolites of fungi which grow on plants of agricultural importance, e.g. human and animal food. Because the aflatoxin content of the food can be accumulated by the animal organisms, such food must conform with strict limits set by international regulations. The aim of our work was to determine the aflatoxin content of fish nourished on special foodstuff, and to trace the origin of the food's aflatoxin content. Fish is considered to be an important constituent of a modern healthy diet, and its quality must meet rigorous requirements. We also examined the aflatoxin content (B1, B2, G1, and G2) of fish nourished on the same foodstuff then spiked with the aflatoxins. The determination of aflatoxins can be performed by different techniques. We used overpressured-layer chromatography (OPLC) and high-performance liquid chromatography (HPLC). Both are suitable for determination of the aflatoxin content within the range allowed by Hungarian regulations. Use of OPLC enabled elimination of extra clean-up steps before chromatographic separation and ensured high sample throughput and low operating costs.

Original languageEnglish
Pages (from-to)383-387
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume12
Issue number5
Publication statusPublished - Sep 1 1999

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Keywords

  • Aflatoxins B, B, G, G
  • Fish
  • HPLC
  • Over-pressured-layer chromatography (OPLC)
  • Quantitative analyses

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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