The role of osteopontin expression in melanoma progression

Timea Kiss, Szilvia Ecsedi, Laura Vizkeleti, Viktoria Koroknai, Gabriella Emri, Nora Kovács, R. Ádány, M. Balázs

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

It was shown that osteopontin (OPN), a glycophosphoprotein, plays divergent roles in cancer progression. In addition to multiple intra- and extracellular functions, it facilitates migration of tumour cells, has crucial role in cell adhesion and is associated with increased metastasis formation. In previous studies, we performed global gene expression profiling on a series of primary melanoma samples and found that OPN was significantly overexpressed in ulcerated melanomas. The major purpose of this study was to define OPN expression in primary melanomas with differing biological behaviours. OPN mRNA expression was analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in primary melanoma tissues. Immunohistochemistry was performed using a tissue microarray. Cox regression tests were used for survival analysis. Greater than 50 % of the tissues exhibited high protein expression that was significantly associated with tumour thickness and metastasis. OPN mRNA expression was significantly increased in thicker melanomas and lesions with an ulcerated surface. Increased expression was primarily detected in advanced-stage tumours. A multivariate Cox regression analysis revealed that high OPN expression, tumour thickness and metastasis were significantly associated with reduced relapse-free survival. In summary, high OPN mRNA and protein expression were associated with a less favourable clinical outcome of primary melanoma patients. We determined that OPN is a significant predictive factor for the survival of primary melanoma patients. Based on our and others data, the high expression of OPN may have a crucial stimulatory role in tumour progression and metastasis formation, which, thus, have been proposed as potential targets for cancer diagnosis and therapy.

Original languageEnglish
Pages (from-to)7841-7847
Number of pages7
JournalTumor Biology
Volume36
Issue number10
DOIs
Publication statusPublished - May 7 2015

Fingerprint

Osteopontin
Melanoma
Neoplasms
Neoplasm Metastasis
Messenger RNA
Survival
Gene Expression Profiling
Survival Analysis
Cell Adhesion
Reverse Transcription
Cell Movement
Proteins
Immunohistochemistry
Regression Analysis
Recurrence
Polymerase Chain Reaction

Keywords

  • Melanoma progression
  • Osteopontin
  • Protein expression
  • Tissue microarray

ASJC Scopus subject areas

  • Cancer Research

Cite this

Kiss, T., Ecsedi, S., Vizkeleti, L., Koroknai, V., Emri, G., Kovács, N., ... Balázs, M. (2015). The role of osteopontin expression in melanoma progression. Tumor Biology, 36(10), 7841-7847. https://doi.org/10.1007/s13277-015-3495-y

The role of osteopontin expression in melanoma progression. / Kiss, Timea; Ecsedi, Szilvia; Vizkeleti, Laura; Koroknai, Viktoria; Emri, Gabriella; Kovács, Nora; Ádány, R.; Balázs, M.

In: Tumor Biology, Vol. 36, No. 10, 07.05.2015, p. 7841-7847.

Research output: Contribution to journalArticle

Kiss, T, Ecsedi, S, Vizkeleti, L, Koroknai, V, Emri, G, Kovács, N, Ádány, R & Balázs, M 2015, 'The role of osteopontin expression in melanoma progression', Tumor Biology, vol. 36, no. 10, pp. 7841-7847. https://doi.org/10.1007/s13277-015-3495-y
Kiss T, Ecsedi S, Vizkeleti L, Koroknai V, Emri G, Kovács N et al. The role of osteopontin expression in melanoma progression. Tumor Biology. 2015 May 7;36(10):7841-7847. https://doi.org/10.1007/s13277-015-3495-y
Kiss, Timea ; Ecsedi, Szilvia ; Vizkeleti, Laura ; Koroknai, Viktoria ; Emri, Gabriella ; Kovács, Nora ; Ádány, R. ; Balázs, M. / The role of osteopontin expression in melanoma progression. In: Tumor Biology. 2015 ; Vol. 36, No. 10. pp. 7841-7847.
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