The role of N-methyl-d-aspartate receptors and nitric oxide in cochlear dopamine release

G. Halmos, T. Horváth, G. Polony, Á Fekete, A. Kittel, E. S. Vizi, B. F.A.M. van der Laan, T. Zelles, B. Lendvai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Dopamine (DA) released from lateral olivocochlear (LOC) terminals may have a neuroprotective effect in the cochlea. To explore the role of N-methyl-d-aspartate (NMDA) receptors and nitric oxide (NO) in the modulation of a cochlear DA release, we measured the release of [3H]DA from isolated mouse cochlea in response to the application of NMDA. NMDA at 100 μM significantly increased the electrical-field stimulation-evoked and resting release of DA from the cochlea. The NO donor sodium nitroprusside enhanced the basal outflow of DA but failed to influence the evoked release. The administration of the nitric oxide synthase inhibitor Nω-nitro-l-arginine methyl ester (l-NAME) alone was ineffective, but it significantly inhibited the initial phase of the NMDA-induced elevation of DA outflow, which suggested the role of NO in the NMDA-induced DA release. The DA uptake inhibitor nomifensine increased the electrically evoked release of DA. Nomifensine failed to change the effect of NMDA on the resting or electrically-evoked DA release, which suggested that the uptake mechanism does not play a role in NMDA-evoked and NO-mediated DA release. In summary, we provide evidence that NO can modulate the release of DA from the cochlea following NMDA receptor activation, but does not affect the uptake of DA.

Original languageEnglish
Pages (from-to)796-803
Number of pages8
JournalNeuroscience
Volume154
Issue number2
DOIs
Publication statusPublished - Jun 23 2008

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Keywords

  • NMDA
  • cochlea
  • dopamine
  • lateral olivocochlear efferent
  • nitric oxide
  • uptake

ASJC Scopus subject areas

  • Neuroscience(all)

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