The role of lipopolysaccharide moieties in macrophage response to Escherichia coli

Katalin Eder, C. Vízler, E. Kúsz, Ildiko Karcagi, Hristos Glavinas, G. Balogh, L. Vígh, E. Duda, Zsuzsanna Gyorfy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is the main component of Gram-negative bacteria that - upon infection - activates the host immune system and is crucial in fighting pathogens as well as in the induction of sepsis. In the present study we addressed the question whether the key structural components of LPS equally take part in the activation of different macrophage immune responses. By genomic modifications of Escherichia coli MG1655, we constructed a series of strains harboring complete and truncated forms of LPS in their cell wall. These strains were exposed to RAW 264.7 macrophages, after which phagocytosis, fast release of pre-synthesized TNF and activation of NF-κB signal transduction pathway were quantified. According to our results the core and lipid A moieties are involved in immune recognition. The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-κB. The O-antigen inhibits phagocytosis, leading to immune evasion.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume389
Issue number1
DOIs
Publication statusPublished - Nov 6 2009

Fingerprint

Macrophages
Escherichia coli
Lipopolysaccharides
Lipid A
Chemical activation
Phagocytosis
Immune Evasion
O Antigens
Signal transduction
Macrophage Activation
Immune system
Pathogens
Gram-Negative Bacteria
Cell Wall
Immune System
Signal Transduction
Sepsis
Bacteria
Cells
Infection

Keywords

  • E. coli
  • Innate immune system
  • Lipid rafts
  • Lipopolysaccharide
  • Macrophage functions
  • Receptors

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The role of lipopolysaccharide moieties in macrophage response to Escherichia coli. / Eder, Katalin; Vízler, C.; Kúsz, E.; Karcagi, Ildiko; Glavinas, Hristos; Balogh, G.; Vígh, L.; Duda, E.; Gyorfy, Zsuzsanna.

In: Biochemical and Biophysical Research Communications, Vol. 389, No. 1, 06.11.2009, p. 46-51.

Research output: Contribution to journalArticle

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