The role of lipopolysaccharide moieties in macrophage response to Escherichia coli

Katalin Eder, Csaba Vizler, Erzsebet Kusz, Ildiko Karcagi, Hristos Glavinas, Gabor E. Balogh, Laszlo Vigh, Erno Duda, Zsuzsanna Gyorfy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is the main component of Gram-negative bacteria that - upon infection - activates the host immune system and is crucial in fighting pathogens as well as in the induction of sepsis. In the present study we addressed the question whether the key structural components of LPS equally take part in the activation of different macrophage immune responses. By genomic modifications of Escherichia coli MG1655, we constructed a series of strains harboring complete and truncated forms of LPS in their cell wall. These strains were exposed to RAW 264.7 macrophages, after which phagocytosis, fast release of pre-synthesized TNF and activation of NF-κB signal transduction pathway were quantified. According to our results the core and lipid A moieties are involved in immune recognition. The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-κB. The O-antigen inhibits phagocytosis, leading to immune evasion.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalBiochemical and biophysical research communications
Volume389
Issue number1
DOIs
Publication statusPublished - Nov 6 2009

    Fingerprint

Keywords

  • E. coli
  • Innate immune system
  • Lipid rafts
  • Lipopolysaccharide
  • Macrophage functions
  • Receptors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this