The role of internal calcium-stores in the termination of noradrenaline release during sodium-pump reactivation in peripheral nerves

Tamás L. Török, Péter T. Tóth, Dániel Rácz, Tinh T. Nguyen, Debebe G. Medhin, Awad M. Azzidani, M. Fekete, K. Magyar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The release of [3H]noradrenaline has been measured from the isolated main pulmonary artery of the rabbit in the presence of uptake blockers (cocaine, 3 × 10-5 M; corticosterone, 5 × 10-5 M). K+-removal from Krebs solution increased the release of [3H]NA even in the absence of external calcium. K+-readmission to the external medium terminated the transmitter release. The rate of recovery was much faster than the rate of rise of transmitter release both in the presence and absence of external Ca2+. The main aim of the present study was to examine whether the reactivated Na+-pump per se or the Na+-gradient dependent Ca2+-extrusion and -uptake into the internal calcium-stores is responsible for the termination of transmitter release. In Ca2+-free, 1 mM EGTA containing solution the "K-free" stimulated [3H]NA release was further enhanced by calcium-store releasers (CCCP, 10-5 M; A23187, 3 × 10-6 M). External K+-readmission was effective in abolishing the transmitter release evoked by CCCP and A23187. However when veratrine (10-4 g/ml) was also present the readmitted K+ was ineffective in inhibiting the [3H]NA release although the Na+-pump was fully activated by the elevated level of Na+ inside. The results suggest that the Na+-gradient dependent Ca2+-metabolism of the nerves is responsible for the abolition of transmitter release rather than the Na+-pump reactivation per se.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalNeurochemistry International
Volume10
Issue number2
DOIs
Publication statusPublished - 1987

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Sodium-Potassium-Exchanging ATPase
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Peripheral Nerves
Norepinephrine
Calcimycin
Calcium
Veratrine
Egtazic Acid
Corticosterone
Cocaine
Pulmonary Artery
Rabbits

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

The role of internal calcium-stores in the termination of noradrenaline release during sodium-pump reactivation in peripheral nerves. / Török, Tamás L.; Tóth, Péter T.; Rácz, Dániel; Nguyen, Tinh T.; Medhin, Debebe G.; Azzidani, Awad M.; Fekete, M.; Magyar, K.

In: Neurochemistry International, Vol. 10, No. 2, 1987, p. 205-211.

Research output: Contribution to journalArticle

Török, Tamás L. ; Tóth, Péter T. ; Rácz, Dániel ; Nguyen, Tinh T. ; Medhin, Debebe G. ; Azzidani, Awad M. ; Fekete, M. ; Magyar, K. / The role of internal calcium-stores in the termination of noradrenaline release during sodium-pump reactivation in peripheral nerves. In: Neurochemistry International. 1987 ; Vol. 10, No. 2. pp. 205-211.
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AU - Azzidani, Awad M.

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