The role of intermittent hypoxia on the proliferative inhibition of rat cerebellar astrocytes

Sheng Chun Chiu, Yu Jou Lin, Sung Ying Huang, Chih Feng Lien, Shee Ping Chen, Cheng Yoong Pang, Jian Hong Lin, Kun Ta Yang, F. Gallyas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sleep apnea syndrome, characterized by intermittent hypoxia (IH), is linked with increased oxidative stress. This study investigates the mechanisms underlying IH and the effects of IH-induced oxidative stress on cerebellar astrocytes. Rat primary cerebellar astrocytes were kept in an incubator with an oscillating O2 concentration between 20% and 5% every 30 min for 1-4 days. Although the cell loss increased with the duration, the IH incubation didn't induce apoptosis or necrosis, but rather a G0/G1 cell cycle arrest of cerebellar astro-cytes was noted. ROS accumulation was associated with cell loss during IH. PARP activation, resulting in p21 activation and cyclin D1 degradation was associated with cell cycle G0/G1 arrest of IH-treated cerebellar astrocytes. Our results suggest that IH induces cell loss by enhancing oxidative stress, PARP activation and cell cycle G0/G1 arrest in rat primary cerebellar astrocytes.

Original languageEnglish
Article numbere0132263
JournalPLoS One
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 14 2015

Fingerprint

astrocytes
Astrocytes
Oxidative stress
Rats
hypoxia
G1 Phase Cell Cycle Checkpoints
Chemical activation
rats
Cells
Oxidative Stress
oxidative stress
Cyclin D1
cell cycle
Cell Hypoxia
Incubators
sleep apnea
Sleep Apnea Syndromes
Apoptosis
Degradation
cyclins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chiu, S. C., Lin, Y. J., Huang, S. Y., Lien, C. F., Chen, S. P., Pang, C. Y., ... Gallyas, F. (2015). The role of intermittent hypoxia on the proliferative inhibition of rat cerebellar astrocytes. PLoS One, 10(7), [e0132263]. https://doi.org/10.1371/journal.pone.0132263

The role of intermittent hypoxia on the proliferative inhibition of rat cerebellar astrocytes. / Chiu, Sheng Chun; Lin, Yu Jou; Huang, Sung Ying; Lien, Chih Feng; Chen, Shee Ping; Pang, Cheng Yoong; Lin, Jian Hong; Yang, Kun Ta; Gallyas, F.

In: PLoS One, Vol. 10, No. 7, e0132263, 14.07.2015.

Research output: Contribution to journalArticle

Chiu, SC, Lin, YJ, Huang, SY, Lien, CF, Chen, SP, Pang, CY, Lin, JH, Yang, KT & Gallyas, F 2015, 'The role of intermittent hypoxia on the proliferative inhibition of rat cerebellar astrocytes', PLoS One, vol. 10, no. 7, e0132263. https://doi.org/10.1371/journal.pone.0132263
Chiu, Sheng Chun ; Lin, Yu Jou ; Huang, Sung Ying ; Lien, Chih Feng ; Chen, Shee Ping ; Pang, Cheng Yoong ; Lin, Jian Hong ; Yang, Kun Ta ; Gallyas, F. / The role of intermittent hypoxia on the proliferative inhibition of rat cerebellar astrocytes. In: PLoS One. 2015 ; Vol. 10, No. 7.
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