The role of DNA hypomethylation, histone acetylation and in vivo protein-DNA binding in Epstein-Barr virus-induced CD23 upregulation

Kalman Szenthe, Anita Koroknai, Ferenc Banati, Zoltan Bathori, Hans Helmut Niller, Hans Wolf, Noemi Nagy, Eva Klein, J. Mináróvits, D. Salamon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We analyzed epigenetic marks at the CD23 regulatory regions in well characterized Epstein-Barr virus (EBV)-carrying cell lines covering the major latency types. Bisulfite sequencing showed that DNA methylation is not a major regulator of EBV-induced CD23 transcription, although a wide hypomethylated DNA sequence in the regulatory regions is always present in the cell lines with high CD23 expression. Acetylated histone H3 levels at the CD23b promoter showed strong correlation with CD23b expression, while a weaker correlation could be observed at the CD23a core promoter. DMS in vivo footprinting at the intronic EBV-responsive enhancer and the intermediate-affinity CBF1 site at the CD23a core promoter did not reveal any significant sign of in vivo protein-DNA interactions, despite the presence of strong, characteristic footprints in the same DMS-treated DNA samples at the two CBF1 sites of the LMP2A-promoter. Our in vivo results suggest a minor role for DNA methylation, while a more important role for histone acetylation in the regulation of EBV-induced CD23 expression. Furthermore, our in vivo footprinting results support the complex model of CD23 induction by EBV, rather than a simple model with direct transactivation of CD23 by EBNA-2.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume435
Issue number1
DOIs
Publication statusPublished - May 24 2013

Fingerprint

Acetylation
DNA-Binding Proteins
Human Herpesvirus 4
Viruses
Histones
Up-Regulation
DNA
Nucleic Acid Regulatory Sequences
DNA Methylation
Cells
Cell Line
DNA sequences
Transcription
Epigenomics
Transcriptional Activation
Proteins

Keywords

  • CD23 regulatory regions
  • CpG methylation
  • DMS in vivo footprinting
  • Epstein-Barr virus
  • Histone acetylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The role of DNA hypomethylation, histone acetylation and in vivo protein-DNA binding in Epstein-Barr virus-induced CD23 upregulation. / Szenthe, Kalman; Koroknai, Anita; Banati, Ferenc; Bathori, Zoltan; Helmut Niller, Hans; Wolf, Hans; Nagy, Noemi; Klein, Eva; Mináróvits, J.; Salamon, D.

In: Biochemical and Biophysical Research Communications, Vol. 435, No. 1, 24.05.2013, p. 8-15.

Research output: Contribution to journalArticle

Szenthe, Kalman ; Koroknai, Anita ; Banati, Ferenc ; Bathori, Zoltan ; Helmut Niller, Hans ; Wolf, Hans ; Nagy, Noemi ; Klein, Eva ; Mináróvits, J. ; Salamon, D. / The role of DNA hypomethylation, histone acetylation and in vivo protein-DNA binding in Epstein-Barr virus-induced CD23 upregulation. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 435, No. 1. pp. 8-15.
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