The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis

Petra Misják, Sz. Bősze, Kata Horváti, Mária Pásztói, Krisztina Pálóczi, Marianna C. Holub, Ferenc Szakács, Borbála Aradi, Bence György, Tamás G. Szabó, György Nagy, Tibor T. Glant, Katalin Mikecz, A. Falus, E. Búzás

Research output: Contribution to journalArticle

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Abstract

The P70-84 peptide (also called 5/4E8 epitope) of the human cartilage proteoglycan (PG) aggrecan is the dominant/arthritogenic epitope in both humans and arthritis-prone BALB/c mice (PG-induced arthritis, PGIA). An elevated T cell reactivity was demonstrated to a citrullinated version of the P70-84 epitope in most of the patients with rheumatoid arthritis (RA). The goal of this study was to understand better how a T cell epitope, if citrullinated, may affect antigenicity/arthritogenicity in PGIA, a murine model of RA. T cell reactivity to differentially citrullinated versions of either the human PG aggrecan P70-84 peptide or the corresponding mouse sequence was assessed in peptide or aggrecan-immunized and arthritic BALB/c mice as well as in T cell receptor transgenic mice specific for peptide P70-84 sequence. Peripheral T cell responses were induced by priming BALB/c mice with either the human wild-type or its citrullinated versions. Unexpectedly, priming with the citrullinated self-peptide induced a higher T cell response compared to the wild-type sequence (p

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalImmunology Letters
Volume152
Issue number1
DOIs
Publication statusPublished - Apr 2013

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Aggrecans
T-Lymphocyte Epitopes
Proteoglycans
Arthritis
T-Lymphocytes
Epitopes
Rheumatoid Arthritis
Peptides
T-Cell Antigen Receptor
Transgenic Mice
Cartilage
interleukin-2 (59-72)

Keywords

  • Citrullination
  • Epitope
  • PAD
  • Peptide
  • T cell
  • Thymus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis. / Misják, Petra; Bősze, Sz.; Horváti, Kata; Pásztói, Mária; Pálóczi, Krisztina; Holub, Marianna C.; Szakács, Ferenc; Aradi, Borbála; György, Bence; Szabó, Tamás G.; Nagy, György; Glant, Tibor T.; Mikecz, Katalin; Falus, A.; Búzás, E.

In: Immunology Letters, Vol. 152, No. 1, 04.2013, p. 25-31.

Research output: Contribution to journalArticle

Misják, P, Bősze, S, Horváti, K, Pásztói, M, Pálóczi, K, Holub, MC, Szakács, F, Aradi, B, György, B, Szabó, TG, Nagy, G, Glant, TT, Mikecz, K, Falus, A & Búzás, E 2013, 'The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis', Immunology Letters, vol. 152, no. 1, pp. 25-31. https://doi.org/10.1016/j.imlet.2013.03.005
Misják, Petra ; Bősze, Sz. ; Horváti, Kata ; Pásztói, Mária ; Pálóczi, Krisztina ; Holub, Marianna C. ; Szakács, Ferenc ; Aradi, Borbála ; György, Bence ; Szabó, Tamás G. ; Nagy, György ; Glant, Tibor T. ; Mikecz, Katalin ; Falus, A. ; Búzás, E. / The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis. In: Immunology Letters. 2013 ; Vol. 152, No. 1. pp. 25-31.
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