The role of ascorbic acid and trolox cell pretreatment on transition metal cr(vi)-induced ros generation and cr accumulation: A comparative study

Borut Poljšak, Miklós Pesti, Zoltán Gazdag, József Belagyi, Peter Raspor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Yeast cells can grow in anaerobic and aerobic conditions and are therefore continuously exposed to reactive oxygen species generated as by-products of cellular metabolism. Yeast cells have evolved a number of endogenous antioxidant activities to protect themselves from oxidative stress. Since endogenous antioxidant defense systems cannot be activated prior to Cr(VI)-induced oxidative stress, pretreatment with exogenous antioxidants such as ascorbic acid (vitamin C) and Trolox (a water soluble analogue of vitamin E) against Cr(VI)-induced damage was investigated using yeast Saccharomyces cerevisiae as a model organism. The antioxidative potential of ascorbic acid and Trolox was compared under the same experimental conditions in vivo. The objective of this study was to pretreat yeast cells with the same concentration of ascorbic acid and Trolox in order to increase cell tolerance against reactive chromium intermediates and reactive oxygen species formed during chromium(VI) reduction and compare ascorbic acid and Trolox antioxidative protection. Results indicate the important role of increased cytosol reduction capacity on the modification of Cr(V) formation, increased chromium accumulation, and increased scavenging ability of superoxide anions and hydrogen peroxide. Ascorbic acid and Trolox affects Cr(VI) by decreasing Cr(V) persistence, decreasing the levels of intracellular superoxide anions and peroxide formation, and quenching free radicals formed during Cr(VI) to Cr(III) reduction. Many similarities were observed between acorbic acid and Trolox antioxidative protection against Cr(VI)-induced toxicity. Use of yeast S. cerevisiae as a model organism can improve our understanding of the mechanisms by which higher eukaryotes cope with Cr(VI) toxicity

Original languageEnglish
Title of host publicationTransition Metal Chemistry
Subtitle of host publicationNew Research
PublisherNova Science Publishers, Inc.
Pages173-191
Number of pages19
ISBN (Print)9781604567021
Publication statusPublished - Dec 1 2008

Keywords

  • Ascorbic acid
  • Cr(VI)
  • Reactive oxygen species
  • Trolox
  • Yeast

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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    Poljšak, B., Pesti, M., Gazdag, Z., Belagyi, J., & Raspor, P. (2008). The role of ascorbic acid and trolox cell pretreatment on transition metal cr(vi)-induced ros generation and cr accumulation: A comparative study. In Transition Metal Chemistry: New Research (pp. 173-191). Nova Science Publishers, Inc..