The role of arabidopsis glutathione transferase F9 gene under oxidative stress in seedlings

E. Horváth, Krisztina Bela, Csaba Papdi, Ágnes Gallé, L. Szabados, I. Tari, J. Csiszár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Arabidopsis thaliana contains 54 soluble glutathione transferases (GSTs, EC 2.5.1.18), which are thought to play major roles in oxidative stress responses, but little is known about the function of individual isoenzymes. The role of AtGST phi 9 (GSTF9) in the salt- and salicylic acid response was investigated using 2-week-old Atgstf9 and wild type (Wt) plants. Atgstf9 mutants accumulated more ascorbic acid (AsA) and glutathione (GSH) and had decreased glutathione peroxidase (GPOX) activity under control conditions. Treatment of 2-week-old seedlings with 10-7 M salicylic acid (SA) for 48 h resulted in elevated H2O2 level and enhanced GST activity in Atgstf9 plants, 10-5 M SA treatment enhanced the malondialdehyde and dehydroascorbate contents compared to Wt. 50 and 150 mM NaCl increased the GST activity, AsA and GSH accumulation in Atgstf9 seedlings more pronounced than in Wt plants. We found that the Atgstf9 mutants had altered redox homeostasis under control and stress conditions, in which elevated AsA and GSH levels and modified GST and GPOX activities may play significant role. The half-cell potential values calculated from the concentration of GSH and GSSG indicate that this GST isoenzyme has an important role in the salt stress response.

Original languageEnglish
Pages (from-to)406-418
Number of pages13
JournalActa Biologica Hungarica
Volume66
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

salicylic acid
Oxidative stress
Salicylic Acid
ascorbic acid
Glutathione Transferase
Seedlings
Arabidopsis
Ascorbic Acid
Oxidative Stress
Genes
seedling
Glutathione Peroxidase
Isoenzymes
gene
Salts
salt
Glutathione Disulfide
homeostasis
Malondialdehyde
Oxidation-Reduction

Keywords

  • Arabidopsis thaliana
  • Ascorbic acid
  • Glutathione
  • Glutathione peroxidase activity
  • Glutathione transferase phi 9

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

The role of arabidopsis glutathione transferase F9 gene under oxidative stress in seedlings. / Horváth, E.; Bela, Krisztina; Papdi, Csaba; Gallé, Ágnes; Szabados, L.; Tari, I.; Csiszár, J.

In: Acta Biologica Hungarica, Vol. 66, No. 4, 01.12.2015, p. 406-418.

Research output: Contribution to journalArticle

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