The role of aquatic birds in the environmental dissemination of human pathogenic Giardia duodenalis cysts and Cryptosporidium oocysts in Hungary

J. Plutzer, Barbara Tomor

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Fecal samples were taken from 132 (103 wild and 29 domestic) aquatic birds on selected areas in Hungary from February 2008 to March 2008. Cryptosporidium oocysts and Giardia cysts were purified from the samples and were viewed via fluorescent antibody staining. Molecular detection tools, such as PCR-sequencing and Loop mediated isothermal amplification (LAMP) were used in order to determine the Cryptosporidium species and Giardia duodenalis assemblages present. All together 6 (5.8%) and 6 (5.8%) samples out of the 103 wild bird samples and 4 (13%) and 7 (24%) samples out of the 29 domestic bird samples have been found to be Cryptosporidium and G. duodenalis positive respectively. The results of this study indicate that aquatic ducks, geese, coot and cormorant can play role in the environmental dissemination of human pathogenic Giardia cysts and Cryptosporidium oocysts in Hungary. To our knowledge, this is the first description of Cryptosporidium sp. in Anser fabalis and Anser anser, furthermore Giardia sp. in Fulica atra, A. fabalis and P. carbo and the first PCR-sequence confirmed detection of C. parvum in A. platyrhynchos and F. atra, G. duodenalis Assemblage A in A. strepera and G. duodenalis Assemblage B in A. anser.

Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalParasitology International
Volume58
Issue number3
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Giardia lamblia
Cryptosporidium
Oocysts
Hungary
Birds
Cysts
Giardia
Geese
Polymerase Chain Reaction
Ducks
Charcoal
Staining and Labeling
Antibodies

Keywords

  • Aquatic birds
  • Cryptosporidium
  • Giardia
  • Human pathogen

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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title = "The role of aquatic birds in the environmental dissemination of human pathogenic Giardia duodenalis cysts and Cryptosporidium oocysts in Hungary",
abstract = "Fecal samples were taken from 132 (103 wild and 29 domestic) aquatic birds on selected areas in Hungary from February 2008 to March 2008. Cryptosporidium oocysts and Giardia cysts were purified from the samples and were viewed via fluorescent antibody staining. Molecular detection tools, such as PCR-sequencing and Loop mediated isothermal amplification (LAMP) were used in order to determine the Cryptosporidium species and Giardia duodenalis assemblages present. All together 6 (5.8{\%}) and 6 (5.8{\%}) samples out of the 103 wild bird samples and 4 (13{\%}) and 7 (24{\%}) samples out of the 29 domestic bird samples have been found to be Cryptosporidium and G. duodenalis positive respectively. The results of this study indicate that aquatic ducks, geese, coot and cormorant can play role in the environmental dissemination of human pathogenic Giardia cysts and Cryptosporidium oocysts in Hungary. To our knowledge, this is the first description of Cryptosporidium sp. in Anser fabalis and Anser anser, furthermore Giardia sp. in Fulica atra, A. fabalis and P. carbo and the first PCR-sequence confirmed detection of C. parvum in A. platyrhynchos and F. atra, G. duodenalis Assemblage A in A. strepera and G. duodenalis Assemblage B in A. anser.",
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