The role of γ/δ T-cell receptor-positive cells in pregnancy: Part II

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

PROBLEM: We have previously demonstrated a significantly increased ratio of γ/δ T-cell receptor (TCR)-positive progesterone receptor(PR)-positive cells in the peripheral blood of healthy pregnant women compared to that of recurrent aborters or non-pregnant individuals. Treatment of pregnancy lymphocytes with a pan anti-γ/δ TCR antibody inhibits progesterone-induced blocking factor (PIBF) production, increases natural killer (NK) activity, and alters the cytokine profile. The present study was aimed at investigating the role of the different γ/δ subpopulations in these phenomena. METHOD OF STUDY: Peripherial blood lymphocytes from healthy pregnant women were incubated with either anti-γ1.4 and δ1, or anti-γ9 and δ2 antibodies. The effect of these treatments on PR induction and interleukin (IL)-10 and IL-12 expression were tested by immunocytochemistry. NK activity of anti-γ/δ treated lymphocytes was also determined. RESULTS: In peripheral blood of healthy pregnant women, the most frequently occurring chain combination was γ1.4/δ1, whereas in recurrent aborters, the γ9/δ2 combination was predominant. Treatment of normal pregnancy lymphocytes with a mixture of γ1.4 and δ1 antibodies resulted in a significantly reduced NK activity and increased PR and IL-10 expression, whereas treatment with a mixture of γ9 and δ2 antibodies significantly reduced IL-10 production and slightly increased IL-12 production and NK activity. These data suggest the presence of two functionally distinct subpopulations in the peripheral blood of pregnant women.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number2
Publication statusPublished - 1999

Fingerprint

T-Cell Antigen Receptor
Pregnant Women
Progesterone Receptors
Interleukin-10
Lymphocytes
Pregnancy
Antibodies
Interleukin-12
Population Growth
Therapeutics
Progesterone
Immunohistochemistry
Cytokines

Keywords

  • IL-10
  • NK activity
  • Progesterone
  • γ/δ subpopulations

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

Cite this

@article{167b1969b11b42f5aea198d10e0259a1,
title = "The role of γ/δ T-cell receptor-positive cells in pregnancy: Part II",
abstract = "PROBLEM: We have previously demonstrated a significantly increased ratio of γ/δ T-cell receptor (TCR)-positive progesterone receptor(PR)-positive cells in the peripheral blood of healthy pregnant women compared to that of recurrent aborters or non-pregnant individuals. Treatment of pregnancy lymphocytes with a pan anti-γ/δ TCR antibody inhibits progesterone-induced blocking factor (PIBF) production, increases natural killer (NK) activity, and alters the cytokine profile. The present study was aimed at investigating the role of the different γ/δ subpopulations in these phenomena. METHOD OF STUDY: Peripherial blood lymphocytes from healthy pregnant women were incubated with either anti-γ1.4 and δ1, or anti-γ9 and δ2 antibodies. The effect of these treatments on PR induction and interleukin (IL)-10 and IL-12 expression were tested by immunocytochemistry. NK activity of anti-γ/δ treated lymphocytes was also determined. RESULTS: In peripheral blood of healthy pregnant women, the most frequently occurring chain combination was γ1.4/δ1, whereas in recurrent aborters, the γ9/δ2 combination was predominant. Treatment of normal pregnancy lymphocytes with a mixture of γ1.4 and δ1 antibodies resulted in a significantly reduced NK activity and increased PR and IL-10 expression, whereas treatment with a mixture of γ9 and δ2 antibodies significantly reduced IL-10 production and slightly increased IL-12 production and NK activity. These data suggest the presence of two functionally distinct subpopulations in the peripheral blood of pregnant women.",
keywords = "IL-10, NK activity, Progesterone, γ/δ subpopulations",
author = "A. Barakonyi and B. Polg{\'a}r and J. Szekeres-Barth{\'o}",
year = "1999",
language = "English",
volume = "42",
pages = "83--87",
journal = "American Journal of Reproductive Immunology",
issn = "8755-8920",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - The role of γ/δ T-cell receptor-positive cells in pregnancy

T2 - Part II

AU - Barakonyi, A.

AU - Polgár, B.

AU - Szekeres-Barthó, J.

PY - 1999

Y1 - 1999

N2 - PROBLEM: We have previously demonstrated a significantly increased ratio of γ/δ T-cell receptor (TCR)-positive progesterone receptor(PR)-positive cells in the peripheral blood of healthy pregnant women compared to that of recurrent aborters or non-pregnant individuals. Treatment of pregnancy lymphocytes with a pan anti-γ/δ TCR antibody inhibits progesterone-induced blocking factor (PIBF) production, increases natural killer (NK) activity, and alters the cytokine profile. The present study was aimed at investigating the role of the different γ/δ subpopulations in these phenomena. METHOD OF STUDY: Peripherial blood lymphocytes from healthy pregnant women were incubated with either anti-γ1.4 and δ1, or anti-γ9 and δ2 antibodies. The effect of these treatments on PR induction and interleukin (IL)-10 and IL-12 expression were tested by immunocytochemistry. NK activity of anti-γ/δ treated lymphocytes was also determined. RESULTS: In peripheral blood of healthy pregnant women, the most frequently occurring chain combination was γ1.4/δ1, whereas in recurrent aborters, the γ9/δ2 combination was predominant. Treatment of normal pregnancy lymphocytes with a mixture of γ1.4 and δ1 antibodies resulted in a significantly reduced NK activity and increased PR and IL-10 expression, whereas treatment with a mixture of γ9 and δ2 antibodies significantly reduced IL-10 production and slightly increased IL-12 production and NK activity. These data suggest the presence of two functionally distinct subpopulations in the peripheral blood of pregnant women.

AB - PROBLEM: We have previously demonstrated a significantly increased ratio of γ/δ T-cell receptor (TCR)-positive progesterone receptor(PR)-positive cells in the peripheral blood of healthy pregnant women compared to that of recurrent aborters or non-pregnant individuals. Treatment of pregnancy lymphocytes with a pan anti-γ/δ TCR antibody inhibits progesterone-induced blocking factor (PIBF) production, increases natural killer (NK) activity, and alters the cytokine profile. The present study was aimed at investigating the role of the different γ/δ subpopulations in these phenomena. METHOD OF STUDY: Peripherial blood lymphocytes from healthy pregnant women were incubated with either anti-γ1.4 and δ1, or anti-γ9 and δ2 antibodies. The effect of these treatments on PR induction and interleukin (IL)-10 and IL-12 expression were tested by immunocytochemistry. NK activity of anti-γ/δ treated lymphocytes was also determined. RESULTS: In peripheral blood of healthy pregnant women, the most frequently occurring chain combination was γ1.4/δ1, whereas in recurrent aborters, the γ9/δ2 combination was predominant. Treatment of normal pregnancy lymphocytes with a mixture of γ1.4 and δ1 antibodies resulted in a significantly reduced NK activity and increased PR and IL-10 expression, whereas treatment with a mixture of γ9 and δ2 antibodies significantly reduced IL-10 production and slightly increased IL-12 production and NK activity. These data suggest the presence of two functionally distinct subpopulations in the peripheral blood of pregnant women.

KW - IL-10

KW - NK activity

KW - Progesterone

KW - γ/δ subpopulations

UR - http://www.scopus.com/inward/record.url?scp=0032798837&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032798837&partnerID=8YFLogxK

M3 - Article

C2 - 10476689

AN - SCOPUS:0032798837

VL - 42

SP - 83

EP - 87

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

SN - 8755-8920

IS - 2

ER -