The rne gene and ribonuclease E

A. Miczák, D. Apirion

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cloned rne+ gene complements temperature sensitive RNase E mutations and directs the synthesis of a polypeptide. In vitro the RNA transcribed from the rne gene directs the synthesis of a number of polypeptides, one of which is identical in size to the in vivo product of the rne gene. A rabbit reticulocyte cell free extract programmed with this RNA produced RNase E activity. Thus, it is evident that the rne gene is the structural gene for RNase E. However, the in vivo product of the cloned RNase E gene is more thermolabile than the chromosomal gene product. When cells containing the rne plasmid were treated with chloramphenicol, the pre-existing RNase E became less heat labile with time. This leads to the suggestion that in the cell RNase E undergoes post-translational modification(s).

Original languageEnglish
Pages (from-to)473-479
Number of pages7
JournalBiochimie
Volume75
Issue number6
DOIs
Publication statusPublished - 1993

Fingerprint

Genes
RNA
Peptides
Reticulocytes
Chloramphenicol
Post Translational Protein Processing
ribonuclease E
Cell Extracts
Plasmids
Hot Temperature
Rabbits
Mutation
Temperature

Keywords

  • post-translational changes
  • RNase E
  • temperature sensitivity

ASJC Scopus subject areas

  • Biochemistry

Cite this

The rne gene and ribonuclease E. / Miczák, A.; Apirion, D.

In: Biochimie, Vol. 75, No. 6, 1993, p. 473-479.

Research output: Contribution to journalArticle

Miczák, A. ; Apirion, D. / The rne gene and ribonuclease E. In: Biochimie. 1993 ; Vol. 75, No. 6. pp. 473-479.
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