The Relative Contributions of Various Transcription Factors to the Overall Promoter Strength of the Mouse Ribosomal Protein L30 Gene

Géza Sáfrány, Robert P. Perry

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The promoter of the mouse gene encoding ribosomal protein L30 contains binding sites for four transcription factors; α (RFX‐1), β (GABP), γ and δ (YY‐1/NF‐E1/UCRBP). The relative contributions of these factors to the strength of the rpL30 promoter in vivo and the degree of synergism among the factors was evaluated by transfection experiments using a series of mutant promoters in which one or more of the binding sites was drastically altered to prevent recognition by its cognate factor. Our results indicated that GABP and RFX‐1 are the major determinants of the rpL30 promoter strength, acting synergistically to boost activity more than eightfold over that which occurs in their absence. The contributions of γ and δ became evident only when the promoter was weakened by eliminating the participation of the other factors. Indeed, as the promoter strength was progressively reduced, the contribution of each individual factor increased, implying that the capacity of the general transcription machinery to be stimulated by these factors is saturable. The activity of the rpL30 promoter was significantly diminished when three pyrimidine residues spanning the start site were converted to purines, indicating that the integrity of the oligopyrimidine tract is also a determinant of the transcriptional efficiency. These studies reveal the hierarchy of importance of four transcription factors that govern the expression of the rpL30 gene. Moreover, they define the graduated levels of promoter activity that would result from deficiencies of these factors in any particular cell type. This information may provide a useful paradigm for understanding the transcriptional regulation of other ubiquitously expressed genes.

Original languageEnglish
Pages (from-to)1066-1072
Number of pages7
JournalEuropean Journal of Biochemistry
Volume230
Issue number3
DOIs
Publication statusPublished - Jun 1995

Keywords

  • Ribosome
  • promoter
  • ribosomal protein
  • transcription factor

ASJC Scopus subject areas

  • Biochemistry

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