The regulation of the induction of vascular endothelial growth factor at the onset of diabetes in spontaneously diabetic rats

L. Braun, T. Kardon, Zs Reisz-Porszasz, G. Banhegyi, J. Mandl

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Early induction of VEGF was studied in liver, kidney and lung of spontaneously diabetic rats. Western blot analysis, northern hybridization were applied to show the expression of VEGF in different organs. Radiolabelled hypoxia responsive element (HRE) and cAMP responsive element (CRE) oligonucleotides were assayed by electrophoretic mobility shift (EMSA) or supershift using anti ARNT and anti CREB-1 monoclonal antibodies. An increase in VEGF expression at the level of protein and mRNA was demonstrated at the beginning of the disease. EMSAs showed: a.) a binding of HIF-1 to HRE and/or CRE, b.) in the same time the binding of CREB-1 was detected to both HRE and/or CRE sequences in the liver, kidney and lung of diabetic animals. Based on these in vivo observations it is supposed that HRE and CRE through the interaction between HIF-1 and CREB-1 are equally involved in the regulation of VEGF expression at the onset of diabetes.

Original languageEnglish
Pages (from-to)2533-2542
Number of pages10
JournalLife Sciences
Volume69
Issue number21
DOIs
Publication statusPublished - Oct 12 2001

Keywords

  • ARNT
  • CREB
  • Diabetes
  • HIF-1
  • VEGF
  • cAMP

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'The regulation of the induction of vascular endothelial growth factor at the onset of diabetes in spontaneously diabetic rats'. Together they form a unique fingerprint.

  • Cite this