The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome

I. Németh, D. Boda

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Red blood cells are well equipped to handle intracellular oxidative stress, their membranes are permeable to O2- and H2O2, and in this way they are important regulators of oxygen reactions occurring in their surroundings. The protective effect against reduced oxygen species - generated during the endothelial cell injury of various tissues - is attributed mainly to the glutathione metabolism of red blood cells. The blood concentration of reduced (GSH) and oxidized glutathione (GSSG) was determined by a sensitive method using glutathione reductase in experimental shock syndromes induced by paraquat toxicity, tourniquet ligature and endotoxin in rats, by bleeding and reperfusion in dogs and by transient ligature of thoracic aorta in newborn piglets. Under these conditions the concentration of GSSG was elevated and GSH was lowered, resulting in an increase in the redox ratio: [GSSG/(GSH + GSSG)] x 100 (GSSG/GSH); particularly during reperfusion. Determination of the GSSG/GSH seems to be a reliable index to assess the degree of oxidative stress 'in vivo'.

Original languageEnglish
JournalBiomedica Biochimica Acta
Volume48
Issue number2-3
Publication statusPublished - 1989

Fingerprint

Oxidative stress
Glutathione Disulfide
Glutathione
Shock
Oxidative Stress
Theoretical Models
Blood
Reperfusion
Ligation
Erythrocytes
Cells
Oxygen
Tourniquets
Paraquat
Glutathione Reductase
Endothelial cells
Thoracic Aorta
Metabolism
Endotoxins
Oxidation-Reduction

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{a0d1e339c6444c7a9d0dda115ff1ea59,
title = "The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome",
abstract = "Red blood cells are well equipped to handle intracellular oxidative stress, their membranes are permeable to O2- and H2O2, and in this way they are important regulators of oxygen reactions occurring in their surroundings. The protective effect against reduced oxygen species - generated during the endothelial cell injury of various tissues - is attributed mainly to the glutathione metabolism of red blood cells. The blood concentration of reduced (GSH) and oxidized glutathione (GSSG) was determined by a sensitive method using glutathione reductase in experimental shock syndromes induced by paraquat toxicity, tourniquet ligature and endotoxin in rats, by bleeding and reperfusion in dogs and by transient ligature of thoracic aorta in newborn piglets. Under these conditions the concentration of GSSG was elevated and GSH was lowered, resulting in an increase in the redox ratio: [GSSG/(GSH + GSSG)] x 100 (GSSG/GSH); particularly during reperfusion. Determination of the GSSG/GSH seems to be a reliable index to assess the degree of oxidative stress 'in vivo'.",
author = "I. N{\'e}meth and D. Boda",
year = "1989",
language = "English",
volume = "48",
journal = "Biomedica Biochimica Acta",
issn = "0232-766X",
publisher = "Akademie Verlag GMBH",
number = "2-3",

}

TY - JOUR

T1 - The ratio of oxidized/reduced glutathione as an index of oxidative stress in various experimental models of shock syndrome

AU - Németh, I.

AU - Boda, D.

PY - 1989

Y1 - 1989

N2 - Red blood cells are well equipped to handle intracellular oxidative stress, their membranes are permeable to O2- and H2O2, and in this way they are important regulators of oxygen reactions occurring in their surroundings. The protective effect against reduced oxygen species - generated during the endothelial cell injury of various tissues - is attributed mainly to the glutathione metabolism of red blood cells. The blood concentration of reduced (GSH) and oxidized glutathione (GSSG) was determined by a sensitive method using glutathione reductase in experimental shock syndromes induced by paraquat toxicity, tourniquet ligature and endotoxin in rats, by bleeding and reperfusion in dogs and by transient ligature of thoracic aorta in newborn piglets. Under these conditions the concentration of GSSG was elevated and GSH was lowered, resulting in an increase in the redox ratio: [GSSG/(GSH + GSSG)] x 100 (GSSG/GSH); particularly during reperfusion. Determination of the GSSG/GSH seems to be a reliable index to assess the degree of oxidative stress 'in vivo'.

AB - Red blood cells are well equipped to handle intracellular oxidative stress, their membranes are permeable to O2- and H2O2, and in this way they are important regulators of oxygen reactions occurring in their surroundings. The protective effect against reduced oxygen species - generated during the endothelial cell injury of various tissues - is attributed mainly to the glutathione metabolism of red blood cells. The blood concentration of reduced (GSH) and oxidized glutathione (GSSG) was determined by a sensitive method using glutathione reductase in experimental shock syndromes induced by paraquat toxicity, tourniquet ligature and endotoxin in rats, by bleeding and reperfusion in dogs and by transient ligature of thoracic aorta in newborn piglets. Under these conditions the concentration of GSSG was elevated and GSH was lowered, resulting in an increase in the redox ratio: [GSSG/(GSH + GSSG)] x 100 (GSSG/GSH); particularly during reperfusion. Determination of the GSSG/GSH seems to be a reliable index to assess the degree of oxidative stress 'in vivo'.

UR - http://www.scopus.com/inward/record.url?scp=0024511854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024511854&partnerID=8YFLogxK

M3 - Article

C2 - 2730630

AN - SCOPUS:0024511854

VL - 48

JO - Biomedica Biochimica Acta

JF - Biomedica Biochimica Acta

SN - 0232-766X

IS - 2-3

ER -