The rate of the AMP/adenosine substrate cycle in concanavalin-A-stimulated rat lymphocytes

Z. Szondy, E. A. Newsholme

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Abstract

The effect of adenosine on the metabolism of prelabelled adenine nucleotides was investigated in concanavalin-A-stimulated rat lymphocytes. Adenosine in the presence of the adenosine deaminase inhibitor, deoxycoformycin, caused a 2-fold increase in the ATP concentration. This effect was, in part, counteracted by an increased rate of adenine nucleotide catabolism, which could be explained by a stimulation of AMP deaminase (EC 3.5.4.6). At the same time a continuous rate of labelled adenosine production was found, which was not affected by the increased ATP concentration and which could only be detected by the trapping effect of a high concentration of added unlabelled adenosine. It is concluded that the rate of the substrate cycle between AMP and adenosine is low (1.9 ± 0.2 nmol/h per 107 cells) in comparison to the rate of AMP deamination.

Original languageEnglish
Pages (from-to)739-742
Number of pages4
JournalBiochemical Journal
Volume261
Issue number3
DOIs
Publication statusPublished - Jan 1 1989

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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