The protein-lipid interface: Perspectives from magnetic resonance and crystal structures

Derek Marsh, T. Páli

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Lipid-protein interactions in membranes are dynamic, and consequently are well studied by magnetic resonance spectroscopy. More recently, lipids associated with integral membrane proteins have been resolved in crystals by X-ray diffraction, mostly at cryogenic temperatures. The conformation and chain ordering of lipids in crystals of integral proteins are reviewed here and are compared and contrasted with results from magnetic resonance and with the crystal structures of phospholipid bilayers. Various aspects of spin-label magnetic resonance studies on lipid interactions with single integral proteins are also reviewed: specificity for phosphatidylcholine, competition with local anaesthetics, oligomer formation of single transmembrane helices, and protein-linked lipid chains. Finally, the interactions between integral proteins and peripheral or lipid-linked proteins, as reflected by the lipid-protein interactions in double reconstitutions, are considered.

Original languageEnglish
Pages (from-to)118-141
Number of pages24
JournalBBA - Biomembranes
Volume1666
Issue number1-2
DOIs
Publication statusPublished - Nov 3 2004

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Magnetic resonance
Magnetic Resonance Spectroscopy
Crystal structure
Lipid-Linked Proteins
Lipids
Proteins
Magnetic resonance spectroscopy
Spin Labels
Crystals
Local Anesthetics
Phosphatidylcholines
Oligomers
X-Ray Diffraction
Cryogenics
Conformations
Phospholipids
Membrane Proteins
Membranes
X ray diffraction
Temperature

Keywords

  • Acetylcholine receptor
  • Avidin
  • Biotinyl lipid
  • Cytochrome c
  • Cytochrome oxidase
  • EPR
  • Local anaesthetic
  • Myelin basic protein
  • Myelin proteolipid
  • Order parameter
  • PDC-109
  • Phospholamban
  • Seminal plasma protein
  • Spin-label
  • Torsion angle

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

The protein-lipid interface : Perspectives from magnetic resonance and crystal structures. / Marsh, Derek; Páli, T.

In: BBA - Biomembranes, Vol. 1666, No. 1-2, 03.11.2004, p. 118-141.

Research output: Contribution to journalArticle

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