The presence of CD209 expressing dendritic cells correlates with biofilm positivity in chronic rhinosinusitis with nasal polyposis

T. Karosi, Péter Csomor, Zoltán Hegyi, I. Sziklai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biofilm-positive cases of chronic rhinosinusitis with nasal polyposis (CRSwNP) may form a separate clinical entity, which is characterized by high recurrence rates and resistance against different therapeutic strategies. This can be explained by a special immunologic phenotype. Biofilm existence has been supposed to correlate with increased amount of dendritic cells that are responsible for antigen presentation in CRSwNP. A total of 20 patients with CRSwNP undergoing endoscopic sinus surgery (ESS) were analyzed. The negative control group consisted of ten patients undergoing septoplasty without CRSwNP. Three series of individual nasal polyps and control specimens were processed to hematoxylin-eosin (HE) and Gram staining and to CD209-specific immunofluorescent assay, respectively. Biofilm was detected in 13 of 20 patients (65 %) with CRSwNP and in none of the ten negative controls. The subepithelial layer of biofilm-positive nasal polyps displayed a statistically significant (p <0.001) increase in the numbers of CD209-expressing dendritic cells compared to biofilm-negative specimens. It was found that biofilm detectability showed strong correlation to the architecture of respiratory mucosa and to the dominant inflammatory cell type of the subepithelial layer. Persisting bacterial biofilms may affect the type of antigen presentation and consecutive immune reactions in the subepithelial layer of nasal mucosa. This phenomenon may result in different inflammatory pathways with specific cytokine profile compared to biofilm-negative cases. Co-existence of bacterial biofilms and dominant pattern of dendritic cells suggest a biofilm-associated immunologic phenotype in CRSwNP. This can explain the mucosal changes, functional disorders and therapy resistance featuring CRSwNP.

Original languageEnglish
Pages (from-to)2455-2463
Number of pages9
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume270
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Biofilms
Nose
Dendritic Cells
Nasal Polyps
Antigen Presentation
Phenotype
Respiratory Mucosa
Nasal Mucosa
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Cytokines
Recurrence
Control Groups
Therapeutics

Keywords

  • Biofilm
  • CD209
  • Chronic rhinosinusitis
  • Dendritic cell
  • Nasal polyp

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

The presence of CD209 expressing dendritic cells correlates with biofilm positivity in chronic rhinosinusitis with nasal polyposis. / Karosi, T.; Csomor, Péter; Hegyi, Zoltán; Sziklai, I.

In: European Archives of Oto-Rhino-Laryngology, Vol. 270, No. 9, 09.2013, p. 2455-2463.

Research output: Contribution to journalArticle

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