The PREPL A protein, a new member of the prolyl oligopeptidase family, lacking catalytic activity

Z. Szeltner, I. Alshafee, T. Juhász, R. Parvari, L. Polgár

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The PREPL (previously called KIAA0436) gene encodes a putative serine peptidase from the prolyl oligopeptidase family. A chromosomal deletion involving the PREPL gene leads to a severe syndrome with multiple symptoms. Homology with oligopeptidase B suggested that the enzyme cleaves after an arginine or lysine residue. Several PREPL splice variants have been identified, and a 638-residue variant (PREPL A) was expressed in Escherichia coli and purified. Its secondary structure was similar to that of oligopeptidase B, but differential-scanning calorimetry indicated a higher conformational stability. Dimerization may account for the enhanced stability. Unexpectedly, the PREPL A protein did not cleave peptide substrates containing a P1 basic residue, but did slowly hydrolyse an activated ester substrate, and reacted with diisopropyl fluorophosphate. These results indicated that the catalytic serine is a reactive residue. However, the negligible hydrolytic activity suggests that the function of PREPL A is different from that of the other members of the prolyl oligopeptidase family.

Original languageEnglish
Pages (from-to)2376-2381
Number of pages6
JournalCellular and Molecular Life Sciences
Volume62
Issue number19-20
DOIs
Publication statusPublished - Oct 2005

Fingerprint

oligopeptidase B
prolyl oligopeptidase
fluorophosphate
Serine
Catalyst activity
Genes
Dimerization
Differential Scanning Calorimetry
Substrates
Escherichia coli
Lysine
Arginine
Differential scanning calorimetry
Esters
Proteins
Peptide Hydrolases
Peptides
Enzymes

Keywords

  • Denaturation
  • Dimerization
  • Hydrolytic activity
  • KIAA0436
  • Oligopeptidase B
  • Protein expression and isolation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

The PREPL A protein, a new member of the prolyl oligopeptidase family, lacking catalytic activity. / Szeltner, Z.; Alshafee, I.; Juhász, T.; Parvari, R.; Polgár, L.

In: Cellular and Molecular Life Sciences, Vol. 62, No. 19-20, 10.2005, p. 2376-2381.

Research output: Contribution to journalArticle

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AU - Polgár, L.

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