The pre- and postjunctional components of the neuromuscular effect of antibiotics

E. Vízi, Ijaz A. Chaudhry, Paul l. Goldiner, Yoshio Ohta, Hideo Nagashima, Francis F. Foldes

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The relative contributions of the pre- and postsynaptic components of the myoneural blocking effect of different antibiotics were studied using: (a) a radioactive method that measures selectively the Ca2+-dependent, stimulation evoked, quantally released,3H-acetylcholine (3H-ACh) from the mouse in vitro phrenic nerve-hemidiaphragm preparation without cholinesterase inhibition; (b) measurement of the force of contraction of the indirectly or directly stimulated muscle. The antibiotics studied (neomycin, polymyxin B and lincomycin), reduced the release of3H-ACh evoked by stimulation (18 trains of 40 shocks at 50 Hz) in a concentration dependent manner. While the inhibitory effect of neomycin was inversely related to [Ca2+]o, that of lincomycin was moderately and that of polymyxin B was not affected by increasing [Ca2+]o from 0.75 to 5.0 mM. Similarly, the dtubocurarine (d-Tc) -induced inhibition of the release of3H-ACh was independent of [Ca2+]o. The K-channel blocking agent, 4-aminopyridine (4-AP), enhanced the release of ACh in a concentration dependent manner and prevented the neuromuscular effect of neomycin. However, the neuromuscular effect of polymyxin B and of lincomycin was not affected by 4-AP. Atropine, enhanced the release of3H-ACh. Antibiotics, however, were still able to reduce the release of ACh when the negative muscarinic feedback mechanism of ACh release was eliminated by atropine. Our findings indicate that the antibiotics studied possess both pre - and postsynaptic effects. Presynaptically they reduce the evoked release of ACh; postsynaptically they inhibit muscle contractility. The rank order of presynaptic action is neomycin >polymyxin B >lincomycin.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Anesthesia
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1991

Fingerprint

Neuromuscular Agents
Lincomycin
Polymyxin B
Neomycin
Anti-Bacterial Agents
4-Aminopyridine
Atropine
Framycetin
Muscles
Phrenic Nerve
Cholinesterases
Cholinergic Agents
Acetylcholine
Shock

Keywords

  • acetylcholine release
  • antibiotic
  • diaphragm
  • margin of safety
  • neomycin
  • neuromuscular junction
  • presynaptic effect

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Vízi, E., Chaudhry, I. A., Goldiner, P. L., Ohta, Y., Nagashima, H., & Foldes, F. F. (1991). The pre- and postjunctional components of the neuromuscular effect of antibiotics. Journal of Anesthesia, 5(1), 1-9. https://doi.org/10.1007/s0054010050001

The pre- and postjunctional components of the neuromuscular effect of antibiotics. / Vízi, E.; Chaudhry, Ijaz A.; Goldiner, Paul l.; Ohta, Yoshio; Nagashima, Hideo; Foldes, Francis F.

In: Journal of Anesthesia, Vol. 5, No. 1, 01.1991, p. 1-9.

Research output: Contribution to journalArticle

Vízi, E, Chaudhry, IA, Goldiner, PL, Ohta, Y, Nagashima, H & Foldes, FF 1991, 'The pre- and postjunctional components of the neuromuscular effect of antibiotics', Journal of Anesthesia, vol. 5, no. 1, pp. 1-9. https://doi.org/10.1007/s0054010050001
Vízi, E. ; Chaudhry, Ijaz A. ; Goldiner, Paul l. ; Ohta, Yoshio ; Nagashima, Hideo ; Foldes, Francis F. / The pre- and postjunctional components of the neuromuscular effect of antibiotics. In: Journal of Anesthesia. 1991 ; Vol. 5, No. 1. pp. 1-9.
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