The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases

Z. Bösze, P. Major, I. Baczkó, K. E. Odening, L. Bodrogi, L. Hiripi, A. Varró

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Since the creation of the first transgenic rabbit thirty years ago, pronuclear microinjection remained the single applied method and resulted in numerous important rabbit models of human diseases, including cardiac deficiencies, albeit with low efficiency. For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future. The targeted genome engineering nuclease family, e.g., the zink finger nuclease (ZFN), the transcription activator-like effector nuclease (TALEN) and the newest, clustered regularly interspaced short palindromic repeats (CRISPR) with the CRISPR associated effector protein (CAS), revolutionized the non-mouse transgenesis. The latest gene-targeting technology, the CRISPR/CAS system, was proven to be efficient in rabbit to create multi-gene knockout models. In the future, the number of tailor-made rabbit models produced with one of the above mentioned methods is expected to exponentially increase and to provide adequate models of heart diseases.

Original languageEnglish
JournalProgress in Biophysics and Molecular Biology
DOIs
Publication statusAccepted/In press - Jan 14 2016

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Gene Transfer Techniques
Heart Diseases
Rabbits
Beauty
Gene Knockout Techniques
Gene Targeting
Microinjections
Fingers
Genome
Technology
Proteins

Keywords

  • Designer nucleases
  • Knock-in
  • Knockout
  • Sleeping beauty transposon
  • Transgenic rabbit

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics

Cite this

The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases. / Bösze, Z.; Major, P.; Baczkó, I.; Odening, K. E.; Bodrogi, L.; Hiripi, L.; Varró, A.

In: Progress in Biophysics and Molecular Biology, 14.01.2016.

Research output: Contribution to journalArticle

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AU - Hiripi, L.

AU - Varró, A.

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