The possible role of CD8+/Vα7.2+/CD161++ T (MAIT) and CD8+/Vα7.2+/CD161lo T (MAIT-like) cells in the pathogenesis of early-onset pre-eclampsia

Matyas Meggyes, Julia Szanto, Adrienn Lajko, Balint Farkas, Akos Varnagy, Peter Tamas, Eszter Hantosi, Eva Miko, L. Szereday

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Problem: The objective of this study was to compare the expressions of different immune-checkpoint molecules by MAIT and MAIT-like cells in healthy pregnancy and in early-onset pre-eclampsia. Method of study: Peripheral blood mononuclear cells (PBMC) were stained with monoclonal antibodies to characterize MAIT and MAIT-like cells. Flow cytometric analyses were used to measure PD-1, TIM-3, activation markers, and intracellular perforin expression. Results: We identified CD3+/CD8+/Vα7.2+/CD161++ MAIT cells and a minor cell population characterized by CD3+/CD8+/Vα7.2+/CD161lo surface markers. In measuring the expression of PD-1 receptor, we found a significantly lower expression by MAIT cells in women with early-onset pre-eclampsia. CD69 expression by MAIT cells was significantly elevated in early-onset pre-eclamptic patients. Intracellular perforin content by MAIT and PD-1+ MAIT cells was significantly increased in pre-eclamptic patients compared with healthy individuals. Conclusion: Altered frequency and reduced PD-1 expression combined together with the elevated perforin content of MAIT cells insinuate their potential roles in the pathogenesis of early-onset pre-eclampsia.

Original languageEnglish
Article numbere12805
JournalAmerican Journal of Reproductive Immunology
Volume79
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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Pre-Eclampsia
Perforin
Programmed Cell Death 1 Receptor
Mucosal-Associated Invariant T Cells
Blood Cells
Monoclonal Antibodies
Pregnancy
Population

Keywords

  • early-onset pre-eclampsia
  • MAIT cells
  • PD-1
  • pregnancy
  • TIM-3

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

The possible role of CD8+/Vα7.2+/CD161++ T (MAIT) and CD8+/Vα7.2+/CD161lo T (MAIT-like) cells in the pathogenesis of early-onset pre-eclampsia. / Meggyes, Matyas; Szanto, Julia; Lajko, Adrienn; Farkas, Balint; Varnagy, Akos; Tamas, Peter; Hantosi, Eszter; Miko, Eva; Szereday, L.

In: American Journal of Reproductive Immunology, Vol. 79, No. 2, e12805, 01.02.2018.

Research output: Contribution to journalArticle

Meggyes, Matyas ; Szanto, Julia ; Lajko, Adrienn ; Farkas, Balint ; Varnagy, Akos ; Tamas, Peter ; Hantosi, Eszter ; Miko, Eva ; Szereday, L. / The possible role of CD8+/Vα7.2+/CD161++ T (MAIT) and CD8+/Vα7.2+/CD161lo T (MAIT-like) cells in the pathogenesis of early-onset pre-eclampsia. In: American Journal of Reproductive Immunology. 2018 ; Vol. 79, No. 2.
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abstract = "Problem: The objective of this study was to compare the expressions of different immune-checkpoint molecules by MAIT and MAIT-like cells in healthy pregnancy and in early-onset pre-eclampsia. Method of study: Peripheral blood mononuclear cells (PBMC) were stained with monoclonal antibodies to characterize MAIT and MAIT-like cells. Flow cytometric analyses were used to measure PD-1, TIM-3, activation markers, and intracellular perforin expression. Results: We identified CD3+/CD8+/Vα7.2+/CD161++ MAIT cells and a minor cell population characterized by CD3+/CD8+/Vα7.2+/CD161lo surface markers. In measuring the expression of PD-1 receptor, we found a significantly lower expression by MAIT cells in women with early-onset pre-eclampsia. CD69 expression by MAIT cells was significantly elevated in early-onset pre-eclamptic patients. Intracellular perforin content by MAIT and PD-1+ MAIT cells was significantly increased in pre-eclamptic patients compared with healthy individuals. Conclusion: Altered frequency and reduced PD-1 expression combined together with the elevated perforin content of MAIT cells insinuate their potential roles in the pathogenesis of early-onset pre-eclampsia.",
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AU - Szanto, Julia

AU - Lajko, Adrienn

AU - Farkas, Balint

AU - Varnagy, Akos

AU - Tamas, Peter

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