The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF

Shuling Zhou, Anna Ochalek, Karolina Szczesna, Hasan X. Avci, Julianna Kobolák, Eszter Varga, Mikkel Rasmussen, Bjørn Holst, Susanna Cirera, Poul Hyttel, Kristine K. Freude, András Dinnyés

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5 Citations (Scopus)

Abstract

Neural rosettes derived from human induced pluripotent stem cells (iPSCs) have been claimed to be a highly robust in vitro cellular model for biomedical application. They are able to propagate in vitro in the presence of mitogens, including basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). However, these two mitogens are also involved in anterior-posterior patterning in a gradient dependent manner along the neural tube axis. Here, we compared the regional identity of neural rosette cells and specific neural subtypes of their progeny propagated with low and high concentrations of bFGF and EGF. We observed that low concentrations of bFGF and EGF in the culturing system were able to induce forebrain identity of the neural rosettes and promote subsequent cortical neuronal differentiation. On the contrary, high concentrations of these mitogens stimulate a mid-hindbrain fate of the neural rosettes, resulting in subsequent cholinergic neuron differentiation. Thus, our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells.

Original languageEnglish
Pages (from-to)183-194
Number of pages12
JournalDifferentiation
Volume92
Issue number4
DOIs
Publication statusPublished - Oct 1 2016

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Keywords

  • Cholinergic neurons
  • Forebrain NPCs
  • Glutamatergic neurons
  • Mid-hindbrain NPCs
  • Mitogens
  • Neural rosette

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Zhou, S., Ochalek, A., Szczesna, K., Avci, H. X., Kobolák, J., Varga, E., Rasmussen, M., Holst, B., Cirera, S., Hyttel, P., Freude, K. K., & Dinnyés, A. (2016). The positional identity of iPSC-derived neural progenitor cells along the anterior-posterior axis is controlled in a dosage-dependent manner by bFGF and EGF. Differentiation, 92(4), 183-194. https://doi.org/10.1016/j.diff.2016.06.002