The polysialylated neural cell adhesion molecule promotes neurogenesis in vitro

Laszlo Vutskits, Eduardo Gascon, Eloisa Zgraggen, Jozsef Zoltan Kiss

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A characteristic feature of neurogenic sites in the postnatal brain is the expression of the polysialylated forms of the neural cell adhesion molecule (PSA-NCAM). To investigate the role of PSA-NCAM in generation of neuronal populations, we developed an in vitro model where neurogenesis occurs in primary cortical cultures following serum withdrawal. We show that removal or inactivation of the PSA tail of NCAM in these cultures leads to a significant decrease in the number of newly generated neurons. Similarly, cultures prepared from NCAM knock-out mice exhibit a significantly reduced neurogenesis. Pulse-chase experiments using the proliferation marker BrdU reveal that the lack of PSA does not affect the mitotic rate of neural progenitors but rather, it reduces the early survival of newly generated neurons. These results suggest that, in addition to its role in the migration of neuronal progenitors, PSA-NCAM is required for the adequate survival of these cells.

Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalNeurochemical research
Volume31
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

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Keywords

  • Neuronal survival
  • PSA-NCAM
  • Postnatal neurogenesis
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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