The polyether bistratene A activates protein kinase C-δ and induces growth arrest in HL60 cells

Gareth Griffiths, Bernadette Garrone, Elizabeth Deacon, Penelope Owen, Judit Pongracz, Graham Mead, Arthur Bradwell, Dianne Watters, Janet Lord

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Abstract

Bistratene A (BisA) induced growth arrest in G2/M in HL60 cells. In addition, BisA-treated cells (50 nM for 48 h) became adherent and expressed the adhesion molecule CDI 1c, but did not express the monocyte enzyme α-napthyl acetate esterase or phagocytose complement coated yeasts. BisA activated protein kinase C (PKC)-δ and induced translocation of PKC-δ to the nucleus. This suggests that activation of PKC-δ can induce growth arrest and cell adhesion, but is insufficient to mediate full differentiation of HL60 cells, BisA has potential as a new probe for determining the function of PKC isoenzymes, specifically PKC-δ.

Original languageEnglish
Pages (from-to)802-808
Number of pages7
JournalBiochemical and biophysical research communications
Volume222
Issue number3
DOIs
Publication statusPublished - May 24 1996

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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