Bistratene A (BisA) induced growth arrest in G2/M in HL60 cells. In addition, BisA-treated cells (50 nM for 48 h) became adherent and expressed the adhesion molecule CDI 1c, but did not express the monocyte enzyme α-napthyl acetate esterase or phagocytose complement coated yeasts. BisA activated protein kinase C (PKC)-δ and induced translocation of PKC-δ to the nucleus. This suggests that activation of PKC-δ can induce growth arrest and cell adhesion, but is insufficient to mediate full differentiation of HL60 cells, BisA has potential as a new probe for determining the function of PKC isoenzymes, specifically PKC-δ.
|Number of pages||7|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - May 24 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology