The pleiotropic cell separation mutation spl1-1 is a nucleotide substitution in the internal promoter of the proline tRNACGG gene of Schizosaccharomyces pombe

I. Miklós, Katalin Ludanyi, M. Sipiczki

Research output: Contribution to journalArticle

Abstract

spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNACGG gene SPATRNAPRO.02. Its nucleotide sequence contains a C→T substitution in the region corresponding to the B-box of the putative intragenic promoter and the TψC loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNATGG gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalCurrent Genetics
Volume55
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Schizosaccharomyces
Cell Separation
Proline
Nucleotides
Mutation
Transfer RNA
Genes
Temperature
G2 Phase
Giant Cells
Cell Division
Plasmids

Keywords

  • Cell cycle
  • Cytokinesis
  • Fission yeast
  • Septum
  • Transcription
  • tRNA

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "The pleiotropic cell separation mutation spl1-1 is a nucleotide substitution in the internal promoter of the proline tRNACGG gene of Schizosaccharomyces pombe",
abstract = "spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNACGG gene SPATRNAPRO.02. Its nucleotide sequence contains a C→T substitution in the region corresponding to the B-box of the putative intragenic promoter and the TψC loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNATGG gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.",
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author = "I. Mikl{\'o}s and Katalin Ludanyi and M. Sipiczki",
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T1 - The pleiotropic cell separation mutation spl1-1 is a nucleotide substitution in the internal promoter of the proline tRNACGG gene of Schizosaccharomyces pombe

AU - Miklós, I.

AU - Ludanyi, Katalin

AU - Sipiczki, M.

PY - 2009

Y1 - 2009

N2 - spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNACGG gene SPATRNAPRO.02. Its nucleotide sequence contains a C→T substitution in the region corresponding to the B-box of the putative intragenic promoter and the TψC loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNATGG gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.

AB - spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNACGG gene SPATRNAPRO.02. Its nucleotide sequence contains a C→T substitution in the region corresponding to the B-box of the putative intragenic promoter and the TψC loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNATGG gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.

KW - Cell cycle

KW - Cytokinesis

KW - Fission yeast

KW - Septum

KW - Transcription

KW - tRNA

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