The pleiotropic cell separation mutation spl1-1 is a nucleotide substitution in the internal promoter of the proline tRNACGG gene of Schizosaccharomyces pombe

Ida Miklos, Katalin Ludanyi, Matthias Sipiczki

Research output: Contribution to journalReview article

Abstract

spl1-1 was originally identified as a spontaneous mutation genetically interacting with sep1-1 and cdc4-8 in producing multinucleate syncytia. This study shows that it is allelic with the proline-tRNACGG gene SPATRNAPRO.02. Its nucleotide sequence contains a C→T substitution in the region corresponding to the B-box of the putative intragenic promoter and the TψC loop of the mature tRNA. The substitution drastically reduces the transcription efficiency of the gene and pleiotropically affects numerous cellular processes. spl1-1 cells are temperature sensitive, osmosensitive, bend at higher temperatures, have extended G2 phase and are defective in cell separation (septum cleavage). The proline-tRNATGG gene SPATRNAPRO.01 can partially suppress the spl1-1 mutation when introduced into the cells on a multicopy plasmid. The effect of a mutation in a tRNA gene on cell separation brings a new element into the complexity of the regulation of cell division and its co-ordination with other cellular processes in Schizosaccharomyces pombe.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalCurrent Genetics
Volume55
Issue number5
DOIs
Publication statusPublished - Jul 28 2009

    Fingerprint

Keywords

  • Cell cycle
  • Cytokinesis
  • Fission yeast
  • Septum
  • Transcription
  • tRNA

ASJC Scopus subject areas

  • Genetics

Cite this