The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis

Véronique Boudolf, Kobe Vlieghe, Gerrit T S Beemster, Z. Magyar, Juan Antonio Torres Acosta, Sara Maes, Els Van Der Schueren, Dirk Inzé, Lieven De Veylder

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Transgenic Arabidopsis thaliana plants overproducing the E2Fa-DPa transcription factor have two distinct cell-specific phenotypes: some cells divide ectopically and others are stimulated to endocycle. The decision of cells to undergo extra mitotic divisions has been postulated to depend on the presence of a mitosis-inducing factor (MIF). Plants possess a unique class of cyclin-dependent kinases (CDKs; B-type) for which no ortholog is found in other kingdoms. The peak of CDKB1;1 activity around the G2-M boundary suggested that it might be part of the MIF. Plants that overexpressed a dominant negative allele of CDKB1;1 underwent enhanced endoreduplication, demonstrating that CDKB1;1 activity was required to inhibit the endocycle. Moreover, when the mutant CDKB1;1 allele was overexpressed in an E2Fa-DPa-overProducing background, it enhanced the endoreduplication phenotype, whereas the extra mitotic cell divisions normally induced by E2Fa-DPa were repressed. Surprisingly, CDKB1;1 transcription was controlled by the E2F pathway, as shown by its upregulation in E2Fa-DPa-overproducing plants and mutational analysis of the E2F binding site in the CDKB1;1 promoter. These findings illustrate a cross talking mechanism between the G1-S and G2-M transition points.

Original languageEnglish
Pages (from-to)2683-2692
Number of pages10
JournalPlant Cell
Volume16
Issue number10
DOIs
Publication statusPublished - Oct 2004

Fingerprint

CDC2 Protein Kinase
cyclin-dependent kinase
Cyclin-Dependent Kinases
Transcription
Arabidopsis
Transcription Factors
transcription factors
Binding Sites
Cells
Endoreduplication
endopolyploidy
Mitosis
mitosis
Alleles
cells
alleles
Phenotype
phenotype
Cell Division
cell division

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis. / Boudolf, Véronique; Vlieghe, Kobe; Beemster, Gerrit T S; Magyar, Z.; Torres Acosta, Juan Antonio; Maes, Sara; Van Der Schueren, Els; Inzé, Dirk; De Veylder, Lieven.

In: Plant Cell, Vol. 16, No. 10, 10.2004, p. 2683-2692.

Research output: Contribution to journalArticle

Boudolf, V, Vlieghe, K, Beemster, GTS, Magyar, Z, Torres Acosta, JA, Maes, S, Van Der Schueren, E, Inzé, D & De Veylder, L 2004, 'The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis', Plant Cell, vol. 16, no. 10, pp. 2683-2692. https://doi.org/10.1105/tpc.104.024398
Boudolf, Véronique ; Vlieghe, Kobe ; Beemster, Gerrit T S ; Magyar, Z. ; Torres Acosta, Juan Antonio ; Maes, Sara ; Van Der Schueren, Els ; Inzé, Dirk ; De Veylder, Lieven. / The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis. In: Plant Cell. 2004 ; Vol. 16, No. 10. pp. 2683-2692.
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