The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film

K. Bryl, G. Varo, R. Drabent

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H2O mixture. The bacteriorhodopsin photocycle in the PVA-H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412-O640 and O640-bRa11-trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared.

Original languageEnglish
Pages (from-to)66-70
Number of pages5
JournalFEBS letters
Volume285
Issue number1
DOIs
Publication statusPublished - Jul 8 1991

Keywords

  • Bacteriorhodopsin
  • Oriented system
  • Photocycle
  • Photoelectric signal

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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