The phosphorylation site of Ca2+-dependent protein kinase from alfalfa

Zoltan Olah, Laszlo Bogre, Csaba Lehel, Anna Farago, Janos Seprodi, Denes Dudits

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A 50 kDa, calcium-dependent protein kinase (CDPK) was purified about 1000-fold from cultured cells of alfalfa (Medicago varia) on the basis of its histone H1 phosphorylation activity. The major polypeptide from bovine histone H1 phosphorylated by either animal protein kinase C (PK-C) or by the alfalfa CDPK gave an identical phosphopeptide pattern. The phosphoamino acid determination showed phosphorylation of serine residues in histone H1 by the plant enzyme. Histone-related oligopeptides known to be substrates for animal histone kinases also served as substrates for the alfalfa kinase. Both of the studied peptides (GKKRKRSRKA; AAASFKAKK) inhibited phosphorylation of H1 histones by bovine and alfalfa kinases. The results of competition studies with the nonapeptide (AAASFKAKK), which is a PK-C specific substrate, suggest common features in target recognition between the plant Ca2+-dependent kinase and animal protein kinase C. We also propose that synthetic peptides like AAASFKAKK can be used as a tool to study substrates of plant kinases in crude cell extracts.

Original languageEnglish
Pages (from-to)453-461
Number of pages9
JournalPlant Molecular Biology
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 1989

Fingerprint

Phosphorylation
Medicago sativa
histones
protein kinases
Histones
Protein Kinases
alfalfa
phosphorylation
phosphotransferases (kinases)
calcium
Phosphotransferases
Protein Kinase C
protein kinase C
Animals
Substrates
animal proteins
Peptides
Protamine Kinase
Phosphoamino Acids
Medicago sativa nothosubsp. varia

Keywords

  • alfalfa
  • Ca-dependent protein kinase (CDPK)
  • oligopeptide substrate
  • peptide competition
  • protein kinase C
  • substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The phosphorylation site of Ca2+-dependent protein kinase from alfalfa. / Olah, Zoltan; Bogre, Laszlo; Lehel, Csaba; Farago, Anna; Seprodi, Janos; Dudits, Denes.

In: Plant Molecular Biology, Vol. 12, No. 4, 04.1989, p. 453-461.

Research output: Contribution to journalArticle

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AU - Dudits, Denes

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