The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs)

Csilla Fodor-Dunai, Inka Fricke, Martin Potocký, Dulguun Dorjgotov, Mõnika Domoki, Manuela E. Jurca, Krisztina Ötvös, Viktor Ẑárský, Antje Berken, Attila Fehér

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Abstract

Plant ROP (Rho of plants) proteins form a unique subgroup within the family of Rho-type small G-proteins of eukaryotes. In this paper we demonstrate that the phosphomimetic mutation of a serine residue conserved in all Rho proteins affects the signaling properties of plant ROPs. We found that the S74E mutation in Medicago ROP6 and Arabidopsis ROP4 prevented the binding of these proteins to their plant-specific upstream activator the plant-specific ROP nucleotide exchanger (PRONE)-domain-containing RopGEF (guanine nucleotide exchange factor) protein and abolished the PRONE-mediated nucleotide exchange reaction in vitro. Structural modeling supported the hypothesis that potential phosphorylation of the S74 residue interferes with the binding of the PRONE-domain to the adjacent plant-specific R76 residue which plays an important role in functional ROP-PRONE interaction. Moreover, we show that while the binding of constitutively active MsROP6 to the effector protein RIC (ROP-interactive CRIB-motif-containing protein) was not affected by the S74E mutation, the capability of this mutated protein to bind and activate the RRK1 kinase in vitro was reduced. These observations are in agreement with the morphology of tobacco pollen tubes expressing mutant forms of yellow fluorescent protein (YFP):MsROP6. The S74E mutation in MsROP6 had no influence on pollen tube morphology and attenuated the phenotype of a constitutively active form of MsROP6. The presented Medicago and Arabidopsis data support the notion that the phosphorylation of the serine residue in ROPs corresponding to S74 in Medicago ROP6 could be a general principle for regulating ROP activation and signaling in plants.

Original languageEnglish
Pages (from-to)669-679
Number of pages11
JournalPlant Journal
Volume66
Issue number4
DOIs
Publication statusPublished - May 2011

Fingerprint

serine
Serine
mutation
Mutation
Nucleotides
Medicago
nucleotides
Pollen Tube
Plant Proteins
Arabidopsis
pollen tubes
proteins
Proteins
phosphorylation
Phosphorylation
Guanine Nucleotide Exchange Factors
rho GTP-Binding Proteins
Amino Acid Motifs
Monomeric GTP-Binding Proteins
Eukaryota

Keywords

  • phosphorylation
  • plant-specific ROP nucleotide exchanger
  • pollen tube growth
  • Rho GTPase
  • ROP guanine nucleotide exchange factor
  • ROP interacting receptor-like cytoplasmic kinase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs). / Fodor-Dunai, Csilla; Fricke, Inka; Potocký, Martin; Dorjgotov, Dulguun; Domoki, Mõnika; Jurca, Manuela E.; Ötvös, Krisztina; Ẑárský, Viktor; Berken, Antje; Fehér, Attila.

In: Plant Journal, Vol. 66, No. 4, 05.2011, p. 669-679.

Research output: Contribution to journalArticle

Fodor-Dunai, C, Fricke, I, Potocký, M, Dorjgotov, D, Domoki, M, Jurca, ME, Ötvös, K, Ẑárský, V, Berken, A & Fehér, A 2011, 'The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs)', Plant Journal, vol. 66, no. 4, pp. 669-679. https://doi.org/10.1111/j.1365-313X.2011.04528.x
Fodor-Dunai, Csilla ; Fricke, Inka ; Potocký, Martin ; Dorjgotov, Dulguun ; Domoki, Mõnika ; Jurca, Manuela E. ; Ötvös, Krisztina ; Ẑárský, Viktor ; Berken, Antje ; Fehér, Attila. / The phosphomimetic mutation of an evolutionarily conserved serine residue affects the signaling properties of Rho of plants (ROPs). In: Plant Journal. 2011 ; Vol. 66, No. 4. pp. 669-679.
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AU - Domoki, Mõnika

AU - Jurca, Manuela E.

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KW - ROP interacting receptor-like cytoplasmic kinase

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