The pattern of cytokine gene expression in human colorectal carcinoma

Anna Csiszár, Tamás Szentes, Bea Haraszti, Annamária Balázs, G. Petrányi, Éva Pócsik

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Systemic and local cytokine environment may modulate the immunogenicity of colorectal cancer cells, and affect anti-tumor immune functions of tumor-infiltrating lymphocytes. We therefore investigated cytokine mRNA expression patterns in tumors and peripheral blood mononuclear cells (PBMC) from patients with colorectal adenocarcinoma. IL-2, IFN-γ, tumor necrosis factor-α (TNF-α), IL-4, IL-6, IL-8, IL-10 and IL-1β mRNAs in single cell suspension of freshly isolated colorectal cancer tissue were studied by RT-PCR. Frequencies of cytokine gene expression were compared to those in normal colonic mucosa from tumor patients. The frequencies of IL-2, IFN-γ, IL-4 and IL-10 gene expression were also determined in peripheral blood mononuclear cells from patients with colorectal adenocarcinoma and compared to those of healthy individuals. Tumor samples were more frequently positive for IFN-γ, IL-2, TNF-α and IL-10 gene expression than normal mucosa (p=0.0001, p=0.0118, p=0.001 and p5 cm. The genes for IL-6, IL-β and IL-8 were commonly expressed in both tumor tissue and normal colonic mucosa. IFN-γ transcripts were detected in more PBMC samples from patients with colorectal cancer than those from normal controls (p=0.0449). Thus, colorectal cancer tissue is characterized by a specific pattern of cytokine gene expression. It is likely that multiple interactions between pro- and anti-inflammatory cytokines regulate tumor growth and the functional activity of tumor-infiltrating lymphocytes.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalPathology and Oncology Research
Volume10
Issue number2
Publication statusPublished - 2004

Fingerprint

Colorectal Neoplasms
Cytokines
Gene Expression
Interleukin-10
Interleukin-2
Tumor-Infiltrating Lymphocytes
Blood Cells
Neoplasms
Mucous Membrane
Interleukin-8
Interleukin-4
Interleukin-6
Adenocarcinoma
Tumor Necrosis Factor-alpha
Messenger RNA
Interleukin-1
Suspensions
Anti-Inflammatory Agents
Polymerase Chain Reaction
Growth

Keywords

  • Colorectal carcinoma
  • Cytokine
  • Gene expression
  • RT-PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Csiszár, A., Szentes, T., Haraszti, B., Balázs, A., Petrányi, G., & Pócsik, É. (2004). The pattern of cytokine gene expression in human colorectal carcinoma. Pathology and Oncology Research, 10(2), 109-116.

The pattern of cytokine gene expression in human colorectal carcinoma. / Csiszár, Anna; Szentes, Tamás; Haraszti, Bea; Balázs, Annamária; Petrányi, G.; Pócsik, Éva.

In: Pathology and Oncology Research, Vol. 10, No. 2, 2004, p. 109-116.

Research output: Contribution to journalArticle

Csiszár, A, Szentes, T, Haraszti, B, Balázs, A, Petrányi, G & Pócsik, É 2004, 'The pattern of cytokine gene expression in human colorectal carcinoma', Pathology and Oncology Research, vol. 10, no. 2, pp. 109-116.
Csiszár A, Szentes T, Haraszti B, Balázs A, Petrányi G, Pócsik É. The pattern of cytokine gene expression in human colorectal carcinoma. Pathology and Oncology Research. 2004;10(2):109-116.
Csiszár, Anna ; Szentes, Tamás ; Haraszti, Bea ; Balázs, Annamária ; Petrányi, G. ; Pócsik, Éva. / The pattern of cytokine gene expression in human colorectal carcinoma. In: Pathology and Oncology Research. 2004 ; Vol. 10, No. 2. pp. 109-116.
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