The oxidative stress response of the yeast Candida intermedia to copper, zinc, and selenium exposure

Štefan Fujs, Zoltán Gazdag, Borut Poljšak, Vekoslava Stibilj, Radmila Milačič, M. Pesti, Peter Raspor, Martin Batič

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The yeast Candida intermedia, as a model organism, was used to examine the links between the metal ions exposure, reactive oxygen species generation and oxidative stress response. To estimate intracellular peroxide and superoxide levels, the fluorescence indicators dihydrorhodamine 123 and dihydroethidium were used, respectively. Antioxidant defence systems were investigated by measuring the activity of catalase, glutathione peroxidase and superoxide dismutase and the content of reduced glutathione. Altered superoxide, peroxide, glutathione levels, and the catalase activity were perceived after the treatment with copper. In the samples treated with selenium and zinc the altered peroxide and superoxide levels, and the glutathione peroxidase activity were perceived. The results indicate that the tolerance of the yeast C. intermedia to different metal ions was correlated with the reactive oxygen species generation in the cells and with the efficiency of antioxidative defence systems.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalJournal of Basic Microbiology
Volume45
Issue number2
DOIs
Publication statusPublished - 2005

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Peroxides
Selenium
Candida
Superoxides
Zinc
Copper
Oxidative Stress
Yeasts
Glutathione Peroxidase
Catalase
Glutathione
Reactive Oxygen Species
Metals
Ions
Superoxide Dismutase
Antioxidants
Fluorescence

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

The oxidative stress response of the yeast Candida intermedia to copper, zinc, and selenium exposure. / Fujs, Štefan; Gazdag, Zoltán; Poljšak, Borut; Stibilj, Vekoslava; Milačič, Radmila; Pesti, M.; Raspor, Peter; Batič, Martin.

In: Journal of Basic Microbiology, Vol. 45, No. 2, 2005, p. 125-135.

Research output: Contribution to journalArticle

Fujs, Š, Gazdag, Z, Poljšak, B, Stibilj, V, Milačič, R, Pesti, M, Raspor, P & Batič, M 2005, 'The oxidative stress response of the yeast Candida intermedia to copper, zinc, and selenium exposure', Journal of Basic Microbiology, vol. 45, no. 2, pp. 125-135. https://doi.org/10.1002/jobm.200410480
Fujs, Štefan ; Gazdag, Zoltán ; Poljšak, Borut ; Stibilj, Vekoslava ; Milačič, Radmila ; Pesti, M. ; Raspor, Peter ; Batič, Martin. / The oxidative stress response of the yeast Candida intermedia to copper, zinc, and selenium exposure. In: Journal of Basic Microbiology. 2005 ; Vol. 45, No. 2. pp. 125-135.
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