The osmotically and histamine-induced enhancement of the plasma vasopressin level is diminished by intracerebroventricularly administered orexin in rats

Gyöngyi K. Kis, A. Molnár, Leila Daruka, J. Gardi, Kinga Rákosi, F. László, F. László, C. Varga

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5 Citations (Scopus)

Abstract

The effects of the centrally administered neuropeptides orexin-A on water intake and vasopressin (VP) secretion were studied in male Wistar rats (180-250 g). Different doses (10, 30, and 90 μg/10 μl) of the orexins and the specific orexin receptor-1 (OX 1) antagonist SB 408124 (30 μg/10 μl) were administered intracerebroventricularly (i.c.v.) under anaesthesia, and the water consumption was measured during 6 h. A plasma VP level elevation was induced by histamine (10 mg/kg) or 2.5% NaCl (10 ml/ kg) administered intraperitoneally (i.p.). The plasma VP levels were measured by radioimmunoassay. Increased water consumption was observed after the administration of 30 μg/10 μl orexin-A. There were no changes in basal VP secretion after the administration of different doses of the orexins. A significant increase in plasma VP concentration was detected following histamine administration. After 2.5% NaCl administration, there was a moderate VP level enhancement. Intracerebroventricularly administered orexin-A (30 μg/10 μl) blocked the VP level increase induced by either histamine or 2.5% NaCl administration. The inhibitory effects were prevented by the specific OX 1 receptor antagonist. In conclusion, the orexins increased water consumption. After 30 μg/10 μl orexin-A administration, the polydipsia was more pronounced. The OX 1 receptor antagonist significantly decreased the polydipsia. Histamine or hyperosmotic VP release enhancement was blocked by previously administered orexin. This inhibition was not observed following OX 1 receptor antagonist administration. Our results suggest that the effects of the orexins on water consumption or blockade of the histamine and osmosisinduced VP level increase are mediated by the OX 1 receptor.

Original languageEnglish
Pages (from-to)531-536
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Volume463
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Vasopressins
Histamine
Rats
Orexin Receptors
Plasmas
Drinking
Water
Polydipsia
Orexins
Neuropeptides
Radioimmunoassay
Wistar Rats
Anesthesia

Keywords

  • Histamine
  • Hyperosmosis
  • Orexin
  • Polydipsia
  • SB 408124
  • Vasopressin

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

@article{82789bd24bee4851b39fd1868b902734,
title = "The osmotically and histamine-induced enhancement of the plasma vasopressin level is diminished by intracerebroventricularly administered orexin in rats",
abstract = "The effects of the centrally administered neuropeptides orexin-A on water intake and vasopressin (VP) secretion were studied in male Wistar rats (180-250 g). Different doses (10, 30, and 90 μg/10 μl) of the orexins and the specific orexin receptor-1 (OX 1) antagonist SB 408124 (30 μg/10 μl) were administered intracerebroventricularly (i.c.v.) under anaesthesia, and the water consumption was measured during 6 h. A plasma VP level elevation was induced by histamine (10 mg/kg) or 2.5{\%} NaCl (10 ml/ kg) administered intraperitoneally (i.p.). The plasma VP levels were measured by radioimmunoassay. Increased water consumption was observed after the administration of 30 μg/10 μl orexin-A. There were no changes in basal VP secretion after the administration of different doses of the orexins. A significant increase in plasma VP concentration was detected following histamine administration. After 2.5{\%} NaCl administration, there was a moderate VP level enhancement. Intracerebroventricularly administered orexin-A (30 μg/10 μl) blocked the VP level increase induced by either histamine or 2.5{\%} NaCl administration. The inhibitory effects were prevented by the specific OX 1 receptor antagonist. In conclusion, the orexins increased water consumption. After 30 μg/10 μl orexin-A administration, the polydipsia was more pronounced. The OX 1 receptor antagonist significantly decreased the polydipsia. Histamine or hyperosmotic VP release enhancement was blocked by previously administered orexin. This inhibition was not observed following OX 1 receptor antagonist administration. Our results suggest that the effects of the orexins on water consumption or blockade of the histamine and osmosisinduced VP level increase are mediated by the OX 1 receptor.",
keywords = "Histamine, Hyperosmosis, Orexin, Polydipsia, SB 408124, Vasopressin",
author = "Kis, {Gy{\"o}ngyi K.} and A. Moln{\'a}r and Leila Daruka and J. Gardi and Kinga R{\'a}kosi and F. L{\'a}szl{\'o} and F. L{\'a}szl{\'o} and C. Varga",
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TY - JOUR

T1 - The osmotically and histamine-induced enhancement of the plasma vasopressin level is diminished by intracerebroventricularly administered orexin in rats

AU - Kis, Gyöngyi K.

AU - Molnár, A.

AU - Daruka, Leila

AU - Gardi, J.

AU - Rákosi, Kinga

AU - László, F.

AU - László, F.

AU - Varga, C.

PY - 2012/4

Y1 - 2012/4

N2 - The effects of the centrally administered neuropeptides orexin-A on water intake and vasopressin (VP) secretion were studied in male Wistar rats (180-250 g). Different doses (10, 30, and 90 μg/10 μl) of the orexins and the specific orexin receptor-1 (OX 1) antagonist SB 408124 (30 μg/10 μl) were administered intracerebroventricularly (i.c.v.) under anaesthesia, and the water consumption was measured during 6 h. A plasma VP level elevation was induced by histamine (10 mg/kg) or 2.5% NaCl (10 ml/ kg) administered intraperitoneally (i.p.). The plasma VP levels were measured by radioimmunoassay. Increased water consumption was observed after the administration of 30 μg/10 μl orexin-A. There were no changes in basal VP secretion after the administration of different doses of the orexins. A significant increase in plasma VP concentration was detected following histamine administration. After 2.5% NaCl administration, there was a moderate VP level enhancement. Intracerebroventricularly administered orexin-A (30 μg/10 μl) blocked the VP level increase induced by either histamine or 2.5% NaCl administration. The inhibitory effects were prevented by the specific OX 1 receptor antagonist. In conclusion, the orexins increased water consumption. After 30 μg/10 μl orexin-A administration, the polydipsia was more pronounced. The OX 1 receptor antagonist significantly decreased the polydipsia. Histamine or hyperosmotic VP release enhancement was blocked by previously administered orexin. This inhibition was not observed following OX 1 receptor antagonist administration. Our results suggest that the effects of the orexins on water consumption or blockade of the histamine and osmosisinduced VP level increase are mediated by the OX 1 receptor.

AB - The effects of the centrally administered neuropeptides orexin-A on water intake and vasopressin (VP) secretion were studied in male Wistar rats (180-250 g). Different doses (10, 30, and 90 μg/10 μl) of the orexins and the specific orexin receptor-1 (OX 1) antagonist SB 408124 (30 μg/10 μl) were administered intracerebroventricularly (i.c.v.) under anaesthesia, and the water consumption was measured during 6 h. A plasma VP level elevation was induced by histamine (10 mg/kg) or 2.5% NaCl (10 ml/ kg) administered intraperitoneally (i.p.). The plasma VP levels were measured by radioimmunoassay. Increased water consumption was observed after the administration of 30 μg/10 μl orexin-A. There were no changes in basal VP secretion after the administration of different doses of the orexins. A significant increase in plasma VP concentration was detected following histamine administration. After 2.5% NaCl administration, there was a moderate VP level enhancement. Intracerebroventricularly administered orexin-A (30 μg/10 μl) blocked the VP level increase induced by either histamine or 2.5% NaCl administration. The inhibitory effects were prevented by the specific OX 1 receptor antagonist. In conclusion, the orexins increased water consumption. After 30 μg/10 μl orexin-A administration, the polydipsia was more pronounced. The OX 1 receptor antagonist significantly decreased the polydipsia. Histamine or hyperosmotic VP release enhancement was blocked by previously administered orexin. This inhibition was not observed following OX 1 receptor antagonist administration. Our results suggest that the effects of the orexins on water consumption or blockade of the histamine and osmosisinduced VP level increase are mediated by the OX 1 receptor.

KW - Histamine

KW - Hyperosmosis

KW - Orexin

KW - Polydipsia

KW - SB 408124

KW - Vasopressin

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DO - 10.1007/s00424-012-1080-4

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