The origin of the dual fluorescence of protonated ellipticine in water

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Abstract

Absorption and fluorescence spectroscopic measurements, as well as isothermal calorimetric titrations showed that the biexponential decay kinetics of protonated ellipticine (EH+) fluorescence in water originates from dimerization. Due to the high equilibrium constant for the association of two EH+ and the intense fluorescence of the dimer, deviation from the exponential emission intensity decay commences below micromolar concentrations. Dimerization must be taken into account when EH+ concentration is determined by spectrophotometry, and when EH+ binding to substrates are studied. The molar absorption coefficients of the monomer and dimer were determined.

Original languageEnglish
Pages (from-to)292-295
Number of pages4
JournalChemical Physics Letters
Volume644
DOIs
Publication statusPublished - Jan 16 2016

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

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