The origin of the dual fluorescence of protonated ellipticine in water

Research output: Contribution to journalArticle

1 Citation (Scopus)


Absorption and fluorescence spectroscopic measurements, as well as isothermal calorimetric titrations showed that the biexponential decay kinetics of protonated ellipticine (EH+) fluorescence in water originates from dimerization. Due to the high equilibrium constant for the association of two EH+ and the intense fluorescence of the dimer, deviation from the exponential emission intensity decay commences below micromolar concentrations. Dimerization must be taken into account when EH+ concentration is determined by spectrophotometry, and when EH+ binding to substrates are studied. The molar absorption coefficients of the monomer and dimer were determined.

Original languageEnglish
Pages (from-to)292-295
Number of pages4
JournalChemical Physics Letters
Publication statusPublished - Jan 16 2016


ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

Cite this