The oocyte spindle is preserved by 1,2-propanediol during slow freezing

Ching Chien Chang, Li Ying Sung, Chih Jen Lin, Hilton I. Kort, Xiangzhong Yang, X. Cindy Tian, P. Nagy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To investigate the specific changes in oocyte spindle subjected to severe challenges of low temperature, as well as to examine the effect of cryoprotectants in preserving oocyte spindle during cryopreservation. Design: In vitro experimental study. Setting: Academic research laboratory. Animal(s): B6D2F1 (C57BL/6 X DBA/2) mice. Intervention(s): Mouse oocytes were cryopreserved using a slow freezing method in a sodium-depleted medium with 1.5 mol/l 1,2-propanediol (PROH) and 0.3 M sucrose. To examine the spindle, oocytes were fixed before, during, and after cryopreservation, and oocytes were analyzed by immunocytochemistry and confocal microscopy. Result(s): The MII spindle was preserved during the slow freezing, because the cryoprotectant PROH was found to support the organization of MII spindle in resisting the subzero temperature. In contrast, the MII spindle was disassembled gradually during the thawing process with or without PROH. Most of the oocytes were able to recover the MII spindle after thawing, but a portion of thawed oocytes could not sustain the meiotic spindle because of parthenogenetic activation. Conclusion(s): 1,2-Propanediol can support the organization of MII spindle to defy the subphysiologic temperature; however, the PROH cannot sustain oocyte spindle structure after the subsequent thawing process.

Original languageEnglish
Pages (from-to)1430-1439
Number of pages10
JournalFertility and Sterility
Volume93
Issue number5
DOIs
Publication statusPublished - Mar 15 2010

Fingerprint

Propylene Glycol
Freezing
Oocytes
Cryopreservation
Temperature
Inbred DBA Mouse
Spindle Apparatus
Confocal Microscopy
Sucrose
Sodium
Immunohistochemistry

Keywords

  • 1,2-propanediol
  • meiotic spindle
  • Oocyte cryopreservation

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Chang, C. C., Sung, L. Y., Lin, C. J., Kort, H. I., Yang, X., Tian, X. C., & Nagy, P. (2010). The oocyte spindle is preserved by 1,2-propanediol during slow freezing. Fertility and Sterility, 93(5), 1430-1439. https://doi.org/10.1016/j.fertnstert.2009.01.106

The oocyte spindle is preserved by 1,2-propanediol during slow freezing. / Chang, Ching Chien; Sung, Li Ying; Lin, Chih Jen; Kort, Hilton I.; Yang, Xiangzhong; Tian, X. Cindy; Nagy, P.

In: Fertility and Sterility, Vol. 93, No. 5, 15.03.2010, p. 1430-1439.

Research output: Contribution to journalArticle

Chang, Ching Chien ; Sung, Li Ying ; Lin, Chih Jen ; Kort, Hilton I. ; Yang, Xiangzhong ; Tian, X. Cindy ; Nagy, P. / The oocyte spindle is preserved by 1,2-propanediol during slow freezing. In: Fertility and Sterility. 2010 ; Vol. 93, No. 5. pp. 1430-1439.
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