The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Hungary

I. Tóbiás, B. Kiss, K. Salánki, L. Palkovics

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Barley-infecting isolates of Wheat dwarf virus (WDV) were collected in the field in the vicinity of the cities Dunakiliti, Heves and Siófok, in Hungary. Viral genomic DNA was amplified by the rolling circle amplification technique, digested with Hind III, cloned into pBSK+ plasmid and sequenced. The clones were of the same size and showed above 99% identity to each other. Based on DNA sequences WDV-D01, WDV-H1 and WDV-H07 isolates showed high identity (94-99%) to isolates of WDV barley strain and Barley dwarf virus and lower identity to Oat dwarf virus (71% identity) and WDV wheat strains (85% identity).

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalCereal Research Communications
Volume38
Issue number1
DOIs
Publication statusPublished - Mar 1 2010

Keywords

  • Barley dwarf virus
  • Barley-infecting isolates
  • Oat dwarf virus
  • Sequence analysis
  • Wheat dwarf virus

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics

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